Cloning, expression and characterization of theta-class glutathione S-transferase from the silkworm, Bombyx mori

Kohji Yamamoto, Pingbo Zhang, Fumio Miake, Nobuhiro Kashige, Yoichi Aso, Yutaka Banno, Hiroshi Fujii

研究成果: ジャーナルへの寄稿記事

59 引用 (Scopus)

抄録

This study focused on glutathione S-transferase (GST), one of the detoxification enzymes, from the silkworm, Bombyx mori (GSTT1). A cDNA encoding a putative GST was amplified by reverse transcriptase-polymerase chain reaction and sequenced. The deduced amino acid sequence revealed 59%, 57% and 56% identities to theta-class GSTs of Musca domestica, Anopheles gambiae and Drosophila melanogaster, respectively. GSTT1 was also estimated to be close to those GSTs in a phylogenetic tree. Recombinant GST (rGSTT1) was functionally overexpressed in Escherichia coli in a soluble form, purified to homogeneity, and characterized. The pH-optimum of rGSTT1 was broad from pH 4 to 9 and rGSTT1 retained more than 75% of its original activity after incubation at pH 5-11. Incubation for 30 min at temperatures below 50°C also affected the activity insignificantly. The Michaelis constant for 1-chloro-2,4-dinitrobenzene was 0.48 mM.

元の言語英語
ページ(範囲)340-346
ページ数7
ジャーナルComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
141
発行部数3
DOI
出版物ステータス出版済み - 7 1 2005

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Bombyx
Cloning
Glutathione Transferase
Organism Cloning
Anopheles gambiae
Houseflies
Dinitrochlorobenzene
Detoxification
Polymerase chain reaction
RNA-Directed DNA Polymerase
Drosophila melanogaster
Reverse Transcriptase Polymerase Chain Reaction
Escherichia coli
Amino Acid Sequence
Complementary DNA
Amino Acids
Temperature
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

これを引用

Cloning, expression and characterization of theta-class glutathione S-transferase from the silkworm, Bombyx mori. / Yamamoto, Kohji; Zhang, Pingbo; Miake, Fumio; Kashige, Nobuhiro; Aso, Yoichi; Banno, Yutaka; Fujii, Hiroshi.

:: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 巻 141, 番号 3, 01.07.2005, p. 340-346.

研究成果: ジャーナルへの寄稿記事

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