Cloning, functional characterization, and expression of a gonadotropin receptor cDNA in the ovary and testis of amago salmon (Oncorhynchus rhodurus)

Yuichi Oba, Toshiaki Hirai, Yasutoshi Yoshiura, Michiyasu Yoshikuni, Hiroshi Kawauchi, Yoshitaka Nagahama

研究成果: ジャーナルへの寄稿学術誌査読

95 被引用数 (Scopus)

抄録

A gonadotropin receptor was cloned from amago salmon (Oncorhynchus rhodurus) ovarian follicles. This receptor (sGTH-R) belongs to the glycoprotein hormone receptor family with a large extracellular and seven-transmembrane domains. Its sequence homology is highest with mammalian LH receptors. Phylogenetic analysis reveals that sGTH-R is grouped with mammalian and chicken FSH and LH receptors, but not with mammalian TSH receptors. sGTH-R is expressed dominantly in the ovary and testis. Functional characterization examined with transiently transfected mammalian cells revealed increased intracellular cAMP level when exposed to mammalian and fish gonadotropins; the most potent hormone was salmon GTH II. These results indicate that the cloned cDNA encodes a functional amago salmon GTH receptor protein.

本文言語英語
ページ(範囲)584-590
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
263
2
DOI
出版ステータス出版済み - 9月 24 1999
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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