Cloning of cDNA for vitellogenin of the parasitoid wasp, Pimpla nipponica (Hymenoptera: Apocrita: Ichneumonidae): Vitellogenin primary structure and evolutionary considerations

Yoshiaki Nose, Jae Min Lee, Takatoshi Ueno, Masatsugu Hatakeyama, Kugao Oishi

研究成果: Contribution to journalArticle査読

52 被引用数 (Scopus)

抄録

The cDNA for vitellogenin (Vg) of the parasitoid wasp Pimpla nipponica (Hymenoptera: Apocrita) was cloned and sequenced. The deduced amino acid sequence with 1807 residues was obtained. The N-terminal 20 amino acids chemically determined for vitellin (Vn) agreed completely with the deduced 20 amino acids that follow the 16 amino acid residues for putative signal peptide. The cDNA clone for the Vg of the turnip sawfly Athalia rosae (Hymenoptera: Symphyta), previously obtained and partially sequenced, was also completely sequenced and the amino acid sequence deduced. Amino acid sequences were compared between these two species and also with known Vg sequences from other insects. Common to all these insects is the presence of two long regions with relatively well-conserved amino acid sequences, one near the N-terminal extending 267-282 residues (including two cysteines at conserved locations), and the other starting at position 450 to 655 and extending 279-283 residues, and of a region at the C-terminal extending some 200 residues (about 250 in Aedes aegypti due to the presence of a serine- rich stretch) with 10 cysteines at conserved locations. A molecular phylogenetic tree was constructed.

本文言語英語
ページ(範囲)1047-1056
ページ数10
ジャーナルInsect Biochemistry and Molecular Biology
27
12
DOI
出版ステータス出版済み - 12 1997

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 昆虫科学

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