Cloning of rat p47phox and comparison with human p47 phox

Michiharu Tanabe, Olof Rådmark, Takashi Watanabe, Akira Shiose, Hideki Sumimoto

研究成果: ジャーナルへの寄稿学術誌査読

2 被引用数 (Scopus)


A cDNA encoding rat p47phox was cloned from rat spleen cDNA library, utilizing rapid amplification of cDNA ends. The open reading frame corresponded to 389 amino acids: It contained the phagocyte oxidase homology domain, two Src homology 3 domains and a proline rich region, all of which are conserved in mammalian p47phox sequences. Rat p47phox displayed the highest degree of identity to mouse p47phox (94%). We expressed and purified rat p41phox as a glutathione S-transferase fusion protein, and found that the rat protein could replace human p47 phox in a cell-free activation system for human NADPH oxidase, giving about half activity. Although rat 12-lipoxygenase interacted with human p47phox in a yeast two-hybrid system, this was not the case for rat p47phox.

ジャーナルDNA Sequence - Journal of DNA Sequencing and Mapping
出版ステータス出版済み - 2月 2005

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 遺伝学
  • 内分泌学


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