Cloning of rat p47phox and comparison with human p47 phox

Michiharu Tanabe, Olof Rådmark, Takashi Watanabe, Akira Shiose, Hideki Sumimoto

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

A cDNA encoding rat p47phox was cloned from rat spleen cDNA library, utilizing rapid amplification of cDNA ends. The open reading frame corresponded to 389 amino acids: It contained the phagocyte oxidase homology domain, two Src homology 3 domains and a proline rich region, all of which are conserved in mammalian p47phox sequences. Rat p47phox displayed the highest degree of identity to mouse p47phox (94%). We expressed and purified rat p41phox as a glutathione S-transferase fusion protein, and found that the rat protein could replace human p47 phox in a cell-free activation system for human NADPH oxidase, giving about half activity. Although rat 12-lipoxygenase interacted with human p47phox in a yeast two-hybrid system, this was not the case for rat p47phox.

本文言語英語
ページ(範囲)65-68
ページ数4
ジャーナルDNA Sequence - Journal of DNA Sequencing and Mapping
16
1
DOI
出版ステータス出版済み - 2 2005

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

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