Co-Delivery of Cell-Wall-Forming enzymes in the same vesicle for coordinated fungal cell wall formation

Martin Schuster, Magdalena Martin-Urdiroz, Yujiro Higuchi, Christian Hacker, Sreedhar Kilaru, Sarah J. Gurr, Gero Steinberg

研究成果: Contribution to journalArticle査読

33 被引用数 (Scopus)

抄録

Fungal cells are surrounded by an extracellular cell wall. This complex matrix of proteins and polysaccharides protects against adverse stresses and determines the shape of fungal cells. The polysaccharides of the fungal wall include 1,3-β-glucan and chitin, which are synthesized by membrane-bound synthases at the growing cell tip. A hallmark of filamentous fungi is the class V chitin synthase, which carries a myosin-motor domain. In the corn smut fungus Ustilago maydis, the myosin-chitin synthase Mcs1 moves to the plasma membrane in secretory vesicles, being delivered by kinesin-1 and myosin-5. The myosin domain of Mcs1 enhances polar secretion by tethering vesicles at the site of exocytosis. It remains elusive, however, how other cell-wall-forming enzymes are delivered and how their activity is coordinated post secretion. Here, we show that the U. maydis class VII chitin synthase and 1,3-β-glucan synthase travel in Mcs1-containing vesicles, and that their apical secretion depends on Mcs1. Once in the plasma membrane, anchorage requires enzyme activity, which suggests co-synthesis of chitin and 1,3-β-glucan polysaccharides at sites of exocytosis. Thus, delivery of cell-wall-forming enzymes in Mcs1 vesicles ensures local foci of fungal cell wall formation.

本文言語英語
論文番号16149
ジャーナルNature Microbiology
1
DOI
出版ステータス出版済み - 8 26 2016

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 免疫学
  • 応用微生物学とバイオテクノロジー
  • 遺伝学
  • 微生物学(医療)
  • 細胞生物学

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