Coat assembly directs v-SNARE concentration into synthetic COPII vesicles

Ken Matsuoka, Yasujiro Morimitsu, Koji Uchida, Randy Schekman

研究成果: Contribution to journalArticle査読

75 被引用数 (Scopus)

抄録

COPII proteins are required to create transport vesicles and to select cargo molecules for transit from the ER. A reconstituted liposome budding reaction was used to detect the capture and concentration of membrane-associated v-SNARE molecules into synthetic COPII vesicles. A novel glutathione-phosphatidyl-ethanolamine conjugate (Glut-PE) was synthesized and incorporated into chemically defined liposomes to provide binding sites for GST hybrid proteins. Large liposomes containing bound cytoplasmic domains of the v-SNAREs, Sec22p or Bos1p, or of the ER resident proteins, Sec12p and Ufe1p, were exposed to COPII proteins and GMP-PNP. v-SNAREs but not resident proteins were concentrated in synthetic COPII vesicles generated from donor liposomes. We conclude that COPII proteins are necessary and sufficient for cargo selection and vesicle morphogenesis.

本文言語英語
ページ(範囲)703-708
ページ数6
ジャーナルMolecular Cell
2
5
DOI
出版ステータス出版済み - 11 1998
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 細胞生物学

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