Colloidal gold-based indirect competitive immunochromatographic assay for rapid detection of bioactive isoflavone glycosides daidzin and genistin in soy products

Seiichi Sakamoto, Gorawit Yusakul, Benyakan Pongkitwitoon, Hiroyuki Tanaka, Satoshi Morimoto

研究成果: Contribution to journalArticle査読

21 被引用数 (Scopus)

抄録

Daidzin (DZ) and genistin (GEN) are two major soy isoflavone glycosides isolated from soybeans. Soy products containing isoflavones have recently been widely accepted for commercial use. However, the Japanese Government has suggested that soy isoflavone intake should be limited because of their estrogenic effects due to their interactions with estrogen receptors. In this study, we established a one-step indirect competitive immunochromatographic assay (ICA) for rapid and sensitive detection of total isoflavone glycosides (DZ and GEN) using gold nanoparticles conjugated with a monoclonal antibody against DZ. This assay was able to be completed in 15 min following the immersion of a test strip in an analyte solution. Furthermore, the limit of detection for the total amount of isoflavone glycosides was ∼125 ng mL-1. Considering that the major soy isoflavone glycosides found in soy products are DZ and GEN, this study demonstrates the potential use of ICA for the assessments of over consumption of isoflavones in soy supplements and foods, which would increase the safe dietary intake of soy products.

本文言語英語
ページ(範囲)191-195
ページ数5
ジャーナルFood Chemistry
194
DOI
出版ステータス出版済み - 8 11 2016

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 食品科学

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