Combination of Polysaccharide Nanofibers Derived from Cellulose and Chitin Promotes the Adhesion, Migration and Proliferation of Mouse Fibroblast Cells

研究成果: ジャーナルへの寄稿学術誌査読

2 被引用数 (Scopus)

抄録

Extracellular matrix (ECM) as a structural and biochemical scaffold to surrounding cells plays significant roles in cell adhesion, migration, proliferation and differentiation. Herein, we show the novel combination of TEMPO-oxidized cellulose nanofiber (TOCNF) and surface-N-deacetylated chitin nanofiber (SDCtNF), respectively, having carboxylate and amine groups on each crystalline surface, for mouse fibroblast cell culture. The TOCNF/SDCtNF composite scaffolds demonstrated characteristic cellular behavior, strongly depending on the molar ratios of carboxylates and amines of polysaccharide NFs. Pure TOCNF substrate exhibited good cell attachment, although intact carboxylate-free CNF made no contribution to cell adhesion. By contrast, pure SDCtNF induced crucial cell aggregation to form spheroids; nevertheless, the combination of TOCNF and SDCtNF enhanced cell attachment and subsequent proliferation. Molecular blend of carboxymethylcellulose and acid-soluble chitosan made nearly no contribution to cell culture behavior. The wound healing assay revealed that the polysaccharide combination markedly promoted skin repair for wound heal-ing. Both of TOCNF and SDCtNF possessed rigid nanofiber nanoarchitectures with native crystalline forms and regularly-repeated functional groups, of which such structural characteristics would provide a potential for developing cell culture scaffolds having ECM functions, possibly promoting good cellular adhesion, migration and growth in the designated cellular microenvironments.

本文言語英語
論文番号402
ジャーナルNanomaterials
12
3
DOI
出版ステータス出版済み - 2月 1 2022

!!!All Science Journal Classification (ASJC) codes

  • 化学工学(全般)
  • 材料科学(全般)

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