Comparative analysis for expressed genes by polymerase chain reaction using module-shuffling primers.

C. Uematsu, J. Nishida, K. Okano, H. Kambara, F. Miura, T. Ito, Y. Sakaki

研究成果: ジャーナルへの寄稿記事

抄録

We have developed a method for comparative analysis of gene expression. It is based on competitive PCR amplification with module-shuffling primers, followed by gel electrophoresis in a fluorescent DNA sequencer. In this method, tagged-cDNA restriction fragments derived from different sources were amplified in one tube at the same amplification efficiency. The method can detect different amounts of each expressed gene, up to difference in amounts of 30%. The method was successfully used for comparative analysis of expressed genes in yeast.

元の言語英語
ページ(範囲)91-92
ページ数2
ジャーナルNucleic acids research. Supplement (2001)
発行部数1
DOI
出版物ステータス出版済み - 1 1 2001
外部発表Yes

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Polymerase Chain Reaction
Genes
Electrophoresis
Complementary DNA
Yeasts
Gels
Gene Expression
DNA

これを引用

Comparative analysis for expressed genes by polymerase chain reaction using module-shuffling primers. / Uematsu, C.; Nishida, J.; Okano, K.; Kambara, H.; Miura, F.; Ito, T.; Sakaki, Y.

:: Nucleic acids research. Supplement (2001), 番号 1, 01.01.2001, p. 91-92.

研究成果: ジャーナルへの寄稿記事

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