Comparative analysis of human Src-family kinase substrate specificity in vitro

Hiroyuki Takeda, Yoshifumi Kawamura, Aya Miura, Masatoshi Mori, Ai Wakamatsu, Jun Ichi Yamamoto, Takao Isogai, Masaki Matsumoto, Keiichi Nakayama, Tohru Natsume, Nobuo Nomura, Naoki Goshima

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

Src family kinases (SFKs) are the earliest known family of tyrosine kinases and are widely thought to play essential roles in cellular signal transduction. Although numerous functional analyses have been performed, no study has analyzed the specificity of all SFKs on an equal platform. To gain a better understanding of SFK phosphorylation, we designed a high-throughput in vitro kinase assay on the subproteome scale using surface plasmon resonance. We reacted each of the 11 human SFKs with 519 substrate proteins, and significant phosphorylation was detected in 33.6% (1921) of the total 5709 kinase-substrate combinations. A large number of novel phosphorylations were included among them. Many substrates were shown to be phosphorylated by multiple SFKs, which might reflect functional complementarity of SFKs. Clustering analysis of phosphorylation results grouped substrates into 10 categories, while the similarity of SFK catalytic specificity exhibited no significant correlation with that of amino acid sequences. In silico predictions of SRC-specific phosphorylation sites were not consistent with experimental results, implying some unknown SRC recognition modes. In an attempt to find biologically meaningful novel substrates, phosphorylation data were integrated with annotation data. The extensive in vitro data obtained in this study would provide valuable clues for further understanding SFK-mediated signal transduction.

元の言語英語
ページ(範囲)5982-5993
ページ数12
ジャーナルJournal of Proteome Research
9
発行部数11
DOI
出版物ステータス出版済み - 11 5 2010

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src-Family Kinases
Substrate Specificity
Phosphorylation
Substrates
Signal transduction
Signal Transduction
Phosphotransferases
Surface Plasmon Resonance
In Vitro Techniques
Surface plasmon resonance
Computer Simulation
Protein-Tyrosine Kinases
Cluster Analysis
Amino Acid Sequence
Assays
Throughput
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

これを引用

Takeda, H., Kawamura, Y., Miura, A., Mori, M., Wakamatsu, A., Yamamoto, J. I., ... Goshima, N. (2010). Comparative analysis of human Src-family kinase substrate specificity in vitro. Journal of Proteome Research, 9(11), 5982-5993. https://doi.org/10.1021/pr100773t

Comparative analysis of human Src-family kinase substrate specificity in vitro. / Takeda, Hiroyuki; Kawamura, Yoshifumi; Miura, Aya; Mori, Masatoshi; Wakamatsu, Ai; Yamamoto, Jun Ichi; Isogai, Takao; Matsumoto, Masaki; Nakayama, Keiichi; Natsume, Tohru; Nomura, Nobuo; Goshima, Naoki.

:: Journal of Proteome Research, 巻 9, 番号 11, 05.11.2010, p. 5982-5993.

研究成果: ジャーナルへの寄稿記事

Takeda, H, Kawamura, Y, Miura, A, Mori, M, Wakamatsu, A, Yamamoto, JI, Isogai, T, Matsumoto, M, Nakayama, K, Natsume, T, Nomura, N & Goshima, N 2010, 'Comparative analysis of human Src-family kinase substrate specificity in vitro', Journal of Proteome Research, 巻. 9, 番号 11, pp. 5982-5993. https://doi.org/10.1021/pr100773t
Takeda H, Kawamura Y, Miura A, Mori M, Wakamatsu A, Yamamoto JI その他. Comparative analysis of human Src-family kinase substrate specificity in vitro. Journal of Proteome Research. 2010 11 5;9(11):5982-5993. https://doi.org/10.1021/pr100773t
Takeda, Hiroyuki ; Kawamura, Yoshifumi ; Miura, Aya ; Mori, Masatoshi ; Wakamatsu, Ai ; Yamamoto, Jun Ichi ; Isogai, Takao ; Matsumoto, Masaki ; Nakayama, Keiichi ; Natsume, Tohru ; Nomura, Nobuo ; Goshima, Naoki. / Comparative analysis of human Src-family kinase substrate specificity in vitro. :: Journal of Proteome Research. 2010 ; 巻 9, 番号 11. pp. 5982-5993.
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