Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection

Mostafa Abdelrahman, Naoyuki Suzumura, Mai Mitoma, Satoshi Matsuo, Takao Ikeuchi, Mitsutaka Mori, Kyoko Murakami, Yukio Ozaki, Matsumoto Masaru, Atsuko Uragami, Akira Kanno

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

Asparagus kiusianus, an important wild relative of cultivated asparagus (A. officinalis), exhibits resistance to stem blight disease caused by Phomopsis asparagi. However, the mechanisms underlying this resistance are not understood and no transcriptomic or genetic resources are available for this species. De novo transcriptome sequencing of A. officinalis and A. kiusianus stems was performed 24 h after inoculation with P. asparagi. In total, 35,259 and 36,321 transcripts were annotated in A. officinalis and A. kiusianus, respectively. 1,027 up-regulated and 752 down-regulated transcripts were differentially expressed in the two Asparagus species. RNA sequencing data were validated using quantitative real-Time reverse transcription PCR. Several defense-related genes including peroxidase 4, cationic peroxidase SPC4-like, pathogenesis-related protein-1-like, and jasmonic acid biosynthesis and signaling-related genes including phospholipase D alpha 1, 12-oxophytodienoate reductase and jasmonate-induced protein 23 KD were up-regulated in A. kiusianus relative to A. officinalis. In addition, infected A. kiusianuns exhibited a substantial increase in jasmonic acid and methyl jasmonate relative to A. officinalis. Peroxidase activity was significantly elevated in infected A. kiusianus compared with infected A. officinalis. Our transcriptomic database provides a resource for identifying novel genes and molecular markers-Associated with Phomopsis disease resistance and will facilitate breeding and improvement of cultivated asparagus varieties.

元の言語英語
記事番号2608
ジャーナルScientific reports
7
発行部数1
DOI
出版物ステータス出版済み - 12 1 2017

Fingerprint

Diaporthe
Asparagus officinalis
transcriptome
asparagus spears
transcriptomics
infection
peroxidase
jasmonic acid
phospholipase D
stems
genes
pathogenesis-related proteins
wild relatives
methyl jasmonate
resistance mechanisms
genetic resources
blight
disease resistance
sequence analysis
reverse transcriptase polymerase chain reaction

All Science Journal Classification (ASJC) codes

  • General

これを引用

Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection. / Abdelrahman, Mostafa; Suzumura, Naoyuki; Mitoma, Mai; Matsuo, Satoshi; Ikeuchi, Takao; Mori, Mitsutaka; Murakami, Kyoko; Ozaki, Yukio; Masaru, Matsumoto; Uragami, Atsuko; Kanno, Akira.

:: Scientific reports, 巻 7, 番号 1, 2608, 01.12.2017.

研究成果: ジャーナルへの寄稿記事

Abdelrahman, Mostafa ; Suzumura, Naoyuki ; Mitoma, Mai ; Matsuo, Satoshi ; Ikeuchi, Takao ; Mori, Mitsutaka ; Murakami, Kyoko ; Ozaki, Yukio ; Masaru, Matsumoto ; Uragami, Atsuko ; Kanno, Akira. / Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection. :: Scientific reports. 2017 ; 巻 7, 番号 1.
@article{66aea17303004ebe81d2f020a3d2ccec,
title = "Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection",
abstract = "Asparagus kiusianus, an important wild relative of cultivated asparagus (A. officinalis), exhibits resistance to stem blight disease caused by Phomopsis asparagi. However, the mechanisms underlying this resistance are not understood and no transcriptomic or genetic resources are available for this species. De novo transcriptome sequencing of A. officinalis and A. kiusianus stems was performed 24 h after inoculation with P. asparagi. In total, 35,259 and 36,321 transcripts were annotated in A. officinalis and A. kiusianus, respectively. 1,027 up-regulated and 752 down-regulated transcripts were differentially expressed in the two Asparagus species. RNA sequencing data were validated using quantitative real-Time reverse transcription PCR. Several defense-related genes including peroxidase 4, cationic peroxidase SPC4-like, pathogenesis-related protein-1-like, and jasmonic acid biosynthesis and signaling-related genes including phospholipase D alpha 1, 12-oxophytodienoate reductase and jasmonate-induced protein 23 KD were up-regulated in A. kiusianus relative to A. officinalis. In addition, infected A. kiusianuns exhibited a substantial increase in jasmonic acid and methyl jasmonate relative to A. officinalis. Peroxidase activity was significantly elevated in infected A. kiusianus compared with infected A. officinalis. Our transcriptomic database provides a resource for identifying novel genes and molecular markers-Associated with Phomopsis disease resistance and will facilitate breeding and improvement of cultivated asparagus varieties.",
author = "Mostafa Abdelrahman and Naoyuki Suzumura and Mai Mitoma and Satoshi Matsuo and Takao Ikeuchi and Mitsutaka Mori and Kyoko Murakami and Yukio Ozaki and Matsumoto Masaru and Atsuko Uragami and Akira Kanno",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-02566-7",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Comparative de novo transcriptome profiles in Asparagus officinalis and A. kiusianus during the early stage of Phomopsis asparagi infection

AU - Abdelrahman, Mostafa

AU - Suzumura, Naoyuki

AU - Mitoma, Mai

AU - Matsuo, Satoshi

AU - Ikeuchi, Takao

AU - Mori, Mitsutaka

AU - Murakami, Kyoko

AU - Ozaki, Yukio

AU - Masaru, Matsumoto

AU - Uragami, Atsuko

AU - Kanno, Akira

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Asparagus kiusianus, an important wild relative of cultivated asparagus (A. officinalis), exhibits resistance to stem blight disease caused by Phomopsis asparagi. However, the mechanisms underlying this resistance are not understood and no transcriptomic or genetic resources are available for this species. De novo transcriptome sequencing of A. officinalis and A. kiusianus stems was performed 24 h after inoculation with P. asparagi. In total, 35,259 and 36,321 transcripts were annotated in A. officinalis and A. kiusianus, respectively. 1,027 up-regulated and 752 down-regulated transcripts were differentially expressed in the two Asparagus species. RNA sequencing data were validated using quantitative real-Time reverse transcription PCR. Several defense-related genes including peroxidase 4, cationic peroxidase SPC4-like, pathogenesis-related protein-1-like, and jasmonic acid biosynthesis and signaling-related genes including phospholipase D alpha 1, 12-oxophytodienoate reductase and jasmonate-induced protein 23 KD were up-regulated in A. kiusianus relative to A. officinalis. In addition, infected A. kiusianuns exhibited a substantial increase in jasmonic acid and methyl jasmonate relative to A. officinalis. Peroxidase activity was significantly elevated in infected A. kiusianus compared with infected A. officinalis. Our transcriptomic database provides a resource for identifying novel genes and molecular markers-Associated with Phomopsis disease resistance and will facilitate breeding and improvement of cultivated asparagus varieties.

AB - Asparagus kiusianus, an important wild relative of cultivated asparagus (A. officinalis), exhibits resistance to stem blight disease caused by Phomopsis asparagi. However, the mechanisms underlying this resistance are not understood and no transcriptomic or genetic resources are available for this species. De novo transcriptome sequencing of A. officinalis and A. kiusianus stems was performed 24 h after inoculation with P. asparagi. In total, 35,259 and 36,321 transcripts were annotated in A. officinalis and A. kiusianus, respectively. 1,027 up-regulated and 752 down-regulated transcripts were differentially expressed in the two Asparagus species. RNA sequencing data were validated using quantitative real-Time reverse transcription PCR. Several defense-related genes including peroxidase 4, cationic peroxidase SPC4-like, pathogenesis-related protein-1-like, and jasmonic acid biosynthesis and signaling-related genes including phospholipase D alpha 1, 12-oxophytodienoate reductase and jasmonate-induced protein 23 KD were up-regulated in A. kiusianus relative to A. officinalis. In addition, infected A. kiusianuns exhibited a substantial increase in jasmonic acid and methyl jasmonate relative to A. officinalis. Peroxidase activity was significantly elevated in infected A. kiusianus compared with infected A. officinalis. Our transcriptomic database provides a resource for identifying novel genes and molecular markers-Associated with Phomopsis disease resistance and will facilitate breeding and improvement of cultivated asparagus varieties.

UR - http://www.scopus.com/inward/record.url?scp=85020225225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020225225&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-02566-7

DO - 10.1038/s41598-017-02566-7

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 2608

ER -