2,3,4,7,8-Pentachlorodibenzofuran (PenCDF) was intraperitoneally adoministered in a dose of 60 μg/kg once every two weeks (6 times) to three inbred strains of mice chosen from each of the Ah responsive and nonresponsive ones in order to evaluate in more detail the segregation of the toxicity of PenCDF with the Ah genotype. Results obtained in this work were as follows : (1) Significant changes in the weight of the body, liver, thymus and kidneys were seen in some of the six strains regardless of the Ah genotypes. (2) Aryl hydrocarbon hydroxylase (AHH) activity was significantly enhanced after the PenCDF treatment in the liver, lungs and kidneys of the six strains. The enzyme inducibility in the kidneys was markedly higher in the Ah responsive strains than the nonresponsive ones. (3) PenCDF significantly decreased the number of both the total and B lymphocytes only in the blood of one Ah responsive (C57BL/6NQdj) and one nonresponsive (DDD:Qdj) strains. (4) In a histopathological examination, remarkable changes due to PenCDF were observed only in the liver of the Ah responsive strains. PenCDF also induced higher level of the mitotic index in the liver cells of C57BL/6NQdj and C3H/HeNQdj (Ah responsive strains) and in those of AKR/JSea (Ah nonresponsive strain). Based on the findings described above, the genetic regulation of toxicity caused by PenCDF is considered not to be simple, and these findings may suggest that some biological factors, including genes other than the Ah locus are involved in the development of PenCDF induced toxicity.
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Environmental Chemistry
- Health, Toxicology and Mutagenesis