Comparative transcriptomic characterization of a new mib mutant allele, mibnn2002, in zebrafish

Keng Po Lai, Jing Woei Li, Chia Hao Hsu, May Su You, Ting Fung Chan, Ka Fai William Tse, Yun Jin Jiang

研究成果: ジャーナルへの寄稿記事

抄録

mibnn2002, identified from an allele screen, shows early segmentation defect and severe cell death phenotypes, which are different from those of other described mib mutant alleles. We have previously reported its defects in somitogenesis and identified its origin of mutation, a large deletion in LG2. The report here is a continuous study, where we applied the bioinformatics analysis to profile the genetic background of mibnn2002 mutants. By comparing the transcriptomic data of mibnn2002 mutants with those of AB wild-type, a total of 1945 differentially expressed genes were identified, including 685 up- and 1260 down-regulated genes. The Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis and Ingenuity Pathway Analysis (IPA) identified the enriched pathways and their related biological functions. Our data further demonstrated that the defects in the somitogenesis were related to the down-regulated segmentation genes, such as foxc1a, smyhc1, myod1 and mylpfa.

元の言語英語
ページ(範囲)51-57
ページ数7
ジャーナルGene
642
DOI
出版物ステータス出版済み - 2 5 2018

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Zebrafish
Alleles
Genes
Computational Biology
Cell Death
Databases
Phenotype
Mutation

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Comparative transcriptomic characterization of a new mib mutant allele, mibnn2002, in zebrafish. / Lai, Keng Po; Li, Jing Woei; Hsu, Chia Hao; You, May Su; Chan, Ting Fung; Tse, Ka Fai William; Jiang, Yun Jin.

:: Gene, 巻 642, 05.02.2018, p. 51-57.

研究成果: ジャーナルへの寄稿記事

Lai, Keng Po ; Li, Jing Woei ; Hsu, Chia Hao ; You, May Su ; Chan, Ting Fung ; Tse, Ka Fai William ; Jiang, Yun Jin. / Comparative transcriptomic characterization of a new mib mutant allele, mibnn2002, in zebrafish. :: Gene. 2018 ; 巻 642. pp. 51-57.
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title = "Comparative transcriptomic characterization of a new mib mutant allele, mibnn2002, in zebrafish",
abstract = "mibnn2002, identified from an allele screen, shows early segmentation defect and severe cell death phenotypes, which are different from those of other described mib mutant alleles. We have previously reported its defects in somitogenesis and identified its origin of mutation, a large deletion in LG2. The report here is a continuous study, where we applied the bioinformatics analysis to profile the genetic background of mibnn2002 mutants. By comparing the transcriptomic data of mibnn2002 mutants with those of AB wild-type, a total of 1945 differentially expressed genes were identified, including 685 up- and 1260 down-regulated genes. The Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis and Ingenuity Pathway Analysis (IPA) identified the enriched pathways and their related biological functions. Our data further demonstrated that the defects in the somitogenesis were related to the down-regulated segmentation genes, such as foxc1a, smyhc1, myod1 and mylpfa.",
author = "Lai, {Keng Po} and Li, {Jing Woei} and Hsu, {Chia Hao} and You, {May Su} and Chan, {Ting Fung} and Tse, {Ka Fai William} and Jiang, {Yun Jin}",
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AU - Lai, Keng Po

AU - Li, Jing Woei

AU - Hsu, Chia Hao

AU - You, May Su

AU - Chan, Ting Fung

AU - Tse, Ka Fai William

AU - Jiang, Yun Jin

PY - 2018/2/5

Y1 - 2018/2/5

N2 - mibnn2002, identified from an allele screen, shows early segmentation defect and severe cell death phenotypes, which are different from those of other described mib mutant alleles. We have previously reported its defects in somitogenesis and identified its origin of mutation, a large deletion in LG2. The report here is a continuous study, where we applied the bioinformatics analysis to profile the genetic background of mibnn2002 mutants. By comparing the transcriptomic data of mibnn2002 mutants with those of AB wild-type, a total of 1945 differentially expressed genes were identified, including 685 up- and 1260 down-regulated genes. The Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis and Ingenuity Pathway Analysis (IPA) identified the enriched pathways and their related biological functions. Our data further demonstrated that the defects in the somitogenesis were related to the down-regulated segmentation genes, such as foxc1a, smyhc1, myod1 and mylpfa.

AB - mibnn2002, identified from an allele screen, shows early segmentation defect and severe cell death phenotypes, which are different from those of other described mib mutant alleles. We have previously reported its defects in somitogenesis and identified its origin of mutation, a large deletion in LG2. The report here is a continuous study, where we applied the bioinformatics analysis to profile the genetic background of mibnn2002 mutants. By comparing the transcriptomic data of mibnn2002 mutants with those of AB wild-type, a total of 1945 differentially expressed genes were identified, including 685 up- and 1260 down-regulated genes. The Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis and Ingenuity Pathway Analysis (IPA) identified the enriched pathways and their related biological functions. Our data further demonstrated that the defects in the somitogenesis were related to the down-regulated segmentation genes, such as foxc1a, smyhc1, myod1 and mylpfa.

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