Comparison of sequential derivatization with concurrent methods for GC/MS-based metabolomics

Hiromi Miyagawa, Takeshi Bamba

研究成果: ジャーナルへの寄稿記事

抄録

The gas chromatography/mass spectrometry (GC/MS)-based metabolomics requires a two-step derivatization procedure consisting of oximation and silylation. However, due to the incomplete derivatization and degeneration of the metabolites, good repeatability is difficult to obtain during the batch derivatization, as the time between completing the derivatization process and GC analysis differs from sample to sample. In this research, we successfully obtained good repeatability for the peak areas of 52 selected metabolites by sequential derivatization and interval injection, in which the oximation and silylation times were maintained at constant values. In addition, the derivatization times and amount of reagents employed were varied to confirm that the optimal derivatization conditions differed for the various metabolites. In conventional batch derivatization, six metabolites, viz. glutamine, glutamic acid, histidine, alanine, asparagine, and tryptophan, exhibited fluctuations in their peak areas. Indeed, we found that for all six metabolites these differences originated from the silylation process, while the variations for glutamine and glutamic acid were related to the oximation process.

元の言語英語
ページ(範囲)160-168
ページ数9
ジャーナルJournal of Bioscience and Bioengineering
127
発行部数2
DOI
出版物ステータス出版済み - 2 1 2019

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Metabolomics
Metabolites
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Glutamine
Glutamic Acid
Asparagine
Histidine
Tryptophan
Alanine
Acids
Injections
Research

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Comparison of sequential derivatization with concurrent methods for GC/MS-based metabolomics. / Miyagawa, Hiromi; Bamba, Takeshi.

:: Journal of Bioscience and Bioengineering, 巻 127, 番号 2, 01.02.2019, p. 160-168.

研究成果: ジャーナルへの寄稿記事

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