Comparison of signal peptides for efficient protein secretion in the baculovirus-silkworm system

Yasuhiko Soejima, Jae Man Lee, Yudai Nagata, Hiroaki Mon, Kazuhiro Iiyama, Hajime Kitano, Michiya Matsuyama, Takahiro Kusakabe

研究成果: Contribution to journalArticle査読

33 被引用数 (Scopus)


The baculovirus-silkworm expression system is widely used as a mass production system for recombinant secretory proteins. However, the final yields of some recombinant proteins are not sufficient for industrial use. In this study, we focused on the signal peptide as a key factor for improving the efficiency of protein production. Endoplasmic reticulum (ER) translocation of newly synthesized proteins is the first stage of the secretion pathway; therefore, the selection of an efficient signal peptide would lead to the efficient secretion of recombinant proteins. The Drosophila Bip and honeybee melittin signal peptides have often been used in this system, but to the best of our knowledge, there has been no study comparing secretion efficiency between exogenous and endogenous signal peptides. In this study we employed signal peptides from 30K Da and SP2 proteins as endogenous signals, and compared secretion efficiency with those of exogenous or synthetic origins. We have found that the endogenous secretory signal from the 30K Da protein is the most efficient for recombinant secretory protein production in the baculovirus-silkworm expression system.

ジャーナルCentral European Journal of Biology
出版ステータス出版済み - 1 2013

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 免疫学および微生物学(全般)
  • 農業および生物科学(全般)


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