Competitive ELISA-like label-free detection of lysozyme by using a fluorescent monomer-doped molecularly imprinted hydrogel

Takuya Kubo, Shunsuke Arimura, Toyohiro Naito, Tomoharu Sano, Koji Otsuka

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

We report on a molecularly imprinted hydrogel for the selective fluorescent detection of a targeting protein. To achieve the fluorescent detection of proteins, a fluorescent functional monomer was synthesized with fluorescein isothiocyanate and allylamine. The monomer (FITC-AA) provided a gradable alteration of the absorption spectrum, and a decrease of the fluorescent intensity due to an interaction with the model protein, lysozyme. Also, the imprinted hydrogel (PI-gel) was simply prepared with FITC-AA, the anionic functional monomers, poly(ethylene glycol) dimethacrylate, and lysozyme by radical photo polymerization. The PI-gel showed selective adsorption for lysozyme and the imprinting factor reached to 2.7. Additionally, the PI-gel indicated a gradual decrease of the fluorescent intensity corresponding to the lysozyme concertation similar to a competitive enzyme-linked immunosorbent assay detection.

本文言語英語
ページ(範囲)1311-1315
ページ数5
ジャーナルanalytical sciences
33
11
DOI
出版ステータス出版済み - 2017
外部発表はい

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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