Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer

Yuka Okada, Yuko Ueshin, Ayako Isotani, Tomoko Saito-Fujita, Hisako Nakashima, Kazushi Kimura, Akira Mizoguchi, Masatsugu Ohora, Yoshiko Mori, Masato Ogata, Robert G. Oshima, Masaru Okabe, Masahito Ikawa

    研究成果: ジャーナルへの寄稿記事

    76 引用 (Scopus)

    抜粋

    Placental dysfunction underlies many complications during pregnancy, and better understanding of gene function during placentation could have considerable clinical relevance. However, the lack of a facile method for placenta-specific gene manipulation has hampered investigation of placental organogenesis and the treatment of placental dysfunction. We showed previously that transduction of fertilized mouse eggs with lentiviral vectors leads to transgene expression in both the fetus and the placenta. Here we report placenta-specific gene incorporation by lentiviral transduction of mouse blastocysts after removal of the zona pellucida. All of the placentas analyzed, but none of the fetuses, were transgenic. Application of this method substantially rescued mice deficient in Ets2, Mapk14 (also known as p38α) and Mapk1 (also known as Erk2) from embryonic lethality caused by placental defects. Ectopic expression of Mapk11 also complemented Mapk14 deficiency during placentation.

    元の言語英語
    ページ(範囲)233-237
    ページ数5
    ジャーナルNature Biotechnology
    25
    発行部数2
    DOI
    出版物ステータス出版済み - 2 1 2007

      フィンガープリント

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology
    • Molecular Medicine
    • Biomedical Engineering

    これを引用

    Okada, Y., Ueshin, Y., Isotani, A., Saito-Fujita, T., Nakashima, H., Kimura, K., ... Ikawa, M. (2007). Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer. Nature Biotechnology, 25(2), 233-237. https://doi.org/10.1038/nbt1280