Complete amino acid sequences of the ribosomal proteins L25, L29 and L31 from the archaebacterium Halobacterium marismortui

Tomomitsu HATAKEYAMA, Makoto KIMURA

研究成果: ジャーナルへの寄稿記事

23 引用 (Scopus)

抄録

Ribosomal proteins were extracted from 50s ribosomal subunits of the archaebacterium Halobacterium marismortui by decreasing the concentration of Mg2+ and K+, and the proteins were separated and purified by ion‐exchange column chromatography on DEAE‐cellulose. Ten proteins were purified to homogeneity and three of these proteins were subjected to sequence analysis. The complete amino acid sequences of the ribosomal proteins L25, L29 and L31 were established by analyses of the peptides obtained by enzymatic digestion with trypsin, Staphylococcus aureus protease, chymotrypsin and lysylendopeptidase. Proteins L25, L29 and L31 consist of 84, 115 and 95 amino acid residues with the molecular masses of 9472 Da, 12293 Da and 10418 Da respectively. A comparison of their sequences with those of other large‐ribosomal‐subunit proteins from other organisms revealed that protein L25 from H. marismortui is homologous to protein L23 from Escherichia coli (34.6%), Bacillus stearothermophilus (41.8%), and tobacco chloroplasts (16.3%) as well as to protein L25 from yeast (38.0%). Proteins L29 and L31 do not appear to be homologous to any other ribosomal proteins whose structures are so far known.

元の言語英語
ページ(範囲)703-711
ページ数9
ジャーナルEuropean Journal of Biochemistry
172
発行部数3
DOI
出版物ステータス出版済み - 3 1988

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Haloarcula marismortui
Archaea
Amino Acid Sequence
Amino Acids
Galectin 3
Proteins
Ribosomal Proteins
Geobacillus stearothermophilus
Ribosome Subunits
Fungal Proteins
Escherichia coli Proteins
Chymotrypsin
Chloroplasts
Trypsin
Column chromatography
Tobacco
Sequence Analysis
Staphylococcus aureus
ribosomal protein L31
ribosomal protein L25

All Science Journal Classification (ASJC) codes

  • Biochemistry

これを引用

Complete amino acid sequences of the ribosomal proteins L25, L29 and L31 from the archaebacterium Halobacterium marismortui. / HATAKEYAMA, Tomomitsu; KIMURA, Makoto.

:: European Journal of Biochemistry, 巻 172, 番号 3, 03.1988, p. 703-711.

研究成果: ジャーナルへの寄稿記事

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