Comprehensive Experimental System for a Promising Model Organism Candidate for Marine Teleosts

Keishi Sakaguchi, Michio Yoneda, Noriyoshi Sakai, Kanako Nakashima, Hajime Kitano, Michiya Matsuyama

研究成果: ジャーナルへの寄稿記事

抄録

A comprehensive experimental system for Japanese anchovy, a promising candidate model organism for marine teleosts, was established. Through the design of a rearing/spawning facility that controls the photoperiod and water temperature, one-cell eggs were continuously obtained shortly after spawning throughout the rearing period. The stages of eggs are indispensable for microinjection experiments, and we developed an efficient and robust microinjection system for the Japanese anchovy. Embryos injected with GFP mRNA showed strong whole-body GFP fluorescence and the survival rates of injected- and non-injected embryos were not significantly different, 87.5% (28 in 32 embryos) and 90.0% (45 in 50 embryos), respectively. We verified that the Tol2 transposon system, which mediates gene transfer in vertebrates, worked efficiently in the Japanese anchovy using the transient transgenesis protocol, with GFP or DsRed as the reporter gene. Finally, we confirmed that genome-editing technologies, namely Transcription Activator-Like Effector Nucleases (TALEN) and Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR)/Cas9, were applicable to the Japanese anchovy. In practice, specific gene-disrupted fishes were generated in the F 1 generation. These results demonstrated the establishment of a basic, yet comprehensive, experimental system, which could be employed to undertake experiments using the Japanese anchovy as a model organism for marine teleost fish.

元の言語英語
記事番号4948
ジャーナルScientific reports
9
発行部数1
DOI
出版物ステータス出版済み - 12 1 2019

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Aquatic Organisms
Embryonic Structures
Microinjections
Eggs
Fishes
Facility Regulation and Control
Gene Transfer Techniques
Photoperiod
Reporter Genes
Genes
Vertebrates
Survival Rate
Fluorescence
Technology
Messenger RNA
Temperature
Water

All Science Journal Classification (ASJC) codes

  • General

これを引用

Comprehensive Experimental System for a Promising Model Organism Candidate for Marine Teleosts. / Sakaguchi, Keishi; Yoneda, Michio; Sakai, Noriyoshi; Nakashima, Kanako; Kitano, Hajime; Matsuyama, Michiya.

:: Scientific reports, 巻 9, 番号 1, 4948, 01.12.2019.

研究成果: ジャーナルへの寄稿記事

Sakaguchi, Keishi ; Yoneda, Michio ; Sakai, Noriyoshi ; Nakashima, Kanako ; Kitano, Hajime ; Matsuyama, Michiya. / Comprehensive Experimental System for a Promising Model Organism Candidate for Marine Teleosts. :: Scientific reports. 2019 ; 巻 9, 番号 1.
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