Comprehensive identification of substrates for f-box proteins by differential proteomics analysis

Kanae Yumimoto, Masaki Matsumoto, Koji Oyamada, Toshiro Moroishi, Keiichi I. Nakayama

研究成果: ジャーナルへの寄稿記事

25 引用 (Scopus)

抜粋

Although elucidation of enzyme-substrate relations is fundamental to the advancement of biology, universal approaches to the identification of substrates for a given enzyme have not been established. It is especially difficult to identify substrates for ubiquitin ligases, given that most such substrates are immediately ubiquitylated and degraded as a result of their association with the enzyme. We here describe the development of a new approach, DiPIUS (differential proteomics-based identification of ubiquitylation substrates), to the discovery of substrates for ubiquitin ligases. We applied DiPIUS to Fbxw7α, Skp2, and Fbxl5, three of the most well-characterized F-box proteins, and identified candidate substrates including previously known targets. DiPIUS is thus a powerful tool for unbiased and comprehensive screening for substrates of ubiquitin ligases.

元の言語英語
ページ(範囲)3175-3185
ページ数11
ジャーナルJournal of Proteome Research
11
発行部数6
DOI
出版物ステータス出版済み - 6 1 2012

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

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