Comprehensive validation of T- and B-cell deficiency in rag1-null zebrafish

Implication for the robust innate defense mechanisms of teleosts

Yumie Tokunaga, Masamichi Shirouzu, Ryota Sugahara, Yasutoshi Yoshiura, Ikunari Kiryu, Mitsuru Ototake, Takahiro Nagasawa, Tomonori Somamoto, Miki Nakao

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

rag1 -/- zebrafish have been employed in immunological research as a useful immunodeficient vertebrate model, but with only fragmentary evidence for the lack of functional adaptive immunity. rag1-null zebrafish exhibit differences from their human and murine counterparts in that they can be maintained without any specific pathogen-free conditions. To define the immunodeficient status of rag1 -/- zebrafish, we obtained further functional evidence on T- and B-cell deficiency in the fish at the protein, cellular, and organism levels. Our developed microscale assays provided evidence that rag1 -/- fish do not possess serum IgM protein, that they do not achieve specific protection even after vaccination, and that they cannot induce antigen-specific CTL activity. The mortality rate in non-vaccinated fish suggests that rag1 -/- fish possess innate protection equivalent to that of rag1 -/- fish. Furthermore, poly(I:C)-induced immune responses revealed that the organ that controls anti-viral immunity is shifted from the spleen to the hepatopancreas due to the absence of T- and B-cell function, implying that immune homeostasis may change to an underside mode in rag-null fish. These findings suggest that the teleost relies heavily on innate immunity. Thus, this model could better highlight innate immunity in animals that lack adaptive immunity than mouse models.

元の言語英語
記事番号7536
ジャーナルScientific reports
7
発行部数1
DOI
出版物ステータス出版済み - 12 1 2017

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Zebrafish
Fishes
B-Lymphocytes
Adaptive Immunity
Innate Immunity
Fish Proteins
Hepatopancreas
Specific Pathogen-Free Organisms
Immunoglobulin M
Vertebrates
Blood Proteins
Immunity
Vaccination
Homeostasis
Spleen
Antigens
Mortality
Research

All Science Journal Classification (ASJC) codes

  • General

これを引用

Comprehensive validation of T- and B-cell deficiency in rag1-null zebrafish : Implication for the robust innate defense mechanisms of teleosts. / Tokunaga, Yumie; Shirouzu, Masamichi; Sugahara, Ryota; Yoshiura, Yasutoshi; Kiryu, Ikunari; Ototake, Mitsuru; Nagasawa, Takahiro; Somamoto, Tomonori; Nakao, Miki.

:: Scientific reports, 巻 7, 番号 1, 7536, 01.12.2017.

研究成果: ジャーナルへの寄稿記事

Tokunaga, Yumie ; Shirouzu, Masamichi ; Sugahara, Ryota ; Yoshiura, Yasutoshi ; Kiryu, Ikunari ; Ototake, Mitsuru ; Nagasawa, Takahiro ; Somamoto, Tomonori ; Nakao, Miki. / Comprehensive validation of T- and B-cell deficiency in rag1-null zebrafish : Implication for the robust innate defense mechanisms of teleosts. :: Scientific reports. 2017 ; 巻 7, 番号 1.
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abstract = "rag1 -/- zebrafish have been employed in immunological research as a useful immunodeficient vertebrate model, but with only fragmentary evidence for the lack of functional adaptive immunity. rag1-null zebrafish exhibit differences from their human and murine counterparts in that they can be maintained without any specific pathogen-free conditions. To define the immunodeficient status of rag1 -/- zebrafish, we obtained further functional evidence on T- and B-cell deficiency in the fish at the protein, cellular, and organism levels. Our developed microscale assays provided evidence that rag1 -/- fish do not possess serum IgM protein, that they do not achieve specific protection even after vaccination, and that they cannot induce antigen-specific CTL activity. The mortality rate in non-vaccinated fish suggests that rag1 -/- fish possess innate protection equivalent to that of rag1 -/- fish. Furthermore, poly(I:C)-induced immune responses revealed that the organ that controls anti-viral immunity is shifted from the spleen to the hepatopancreas due to the absence of T- and B-cell function, implying that immune homeostasis may change to an underside mode in rag-null fish. These findings suggest that the teleost relies heavily on innate immunity. Thus, this model could better highlight innate immunity in animals that lack adaptive immunity than mouse models.",
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