TY - JOUR
T1 - Consecutive Sample Injection Analysis in Tube Radial Distribution Chromatography
AU - Takahashi, Yusaku
AU - Nishimura, Kazushi
AU - Tsukagoshi, Kazuhiko
AU - Tsuchiya, Katsumi
AU - Hirota, Ken
AU - Yamashita, Kenichi
AU - Murata, Masaharu
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (B) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan (MEXT) (No. 17H03083).
Publisher Copyright:
© 2021. The Japan Society for Analytical Chemistry
PY - 2021
Y1 - 2021
N2 - Tube radial distribution chromatography based on the tube radial distribution flow, or annular flow, in an open-tubular capillary has been reported, where the annular flow is created through phase-separation multiphase flow. We have proposed the first-ever procedure for consecutive sample injection analysis using chromatography. In basic terms, a commercially available HPLC system could be used with a sample injector (0.2 μL volume) and a fused-silica capillary tube (250 cm long) as a separation column instead of a normal packed one, while the built-in detection cell was replaced by improved on-capillary detection. A ternary mixed solution of water/acetonitrile/ethyl acetate (3:8:4 volume ratio) was delivered into the capillary tube as an eluent at a flow rate of 2.0 μL min–1. Model sample solutions of 1-naphthol and 2,6-naphthalenedisulfonic acid were consecutively analyzed by the present chromatography with a processing rate of 6 samples per hour. Simple and rapid consecutive analysis could be performed because washing and initialization of the separation tube was no longer necessary. The obtained results provide clues to developing new methodologies which combine features of both chromatography (separation) and the flow injection method (consecutive analysis).
AB - Tube radial distribution chromatography based on the tube radial distribution flow, or annular flow, in an open-tubular capillary has been reported, where the annular flow is created through phase-separation multiphase flow. We have proposed the first-ever procedure for consecutive sample injection analysis using chromatography. In basic terms, a commercially available HPLC system could be used with a sample injector (0.2 μL volume) and a fused-silica capillary tube (250 cm long) as a separation column instead of a normal packed one, while the built-in detection cell was replaced by improved on-capillary detection. A ternary mixed solution of water/acetonitrile/ethyl acetate (3:8:4 volume ratio) was delivered into the capillary tube as an eluent at a flow rate of 2.0 μL min–1. Model sample solutions of 1-naphthol and 2,6-naphthalenedisulfonic acid were consecutively analyzed by the present chromatography with a processing rate of 6 samples per hour. Simple and rapid consecutive analysis could be performed because washing and initialization of the separation tube was no longer necessary. The obtained results provide clues to developing new methodologies which combine features of both chromatography (separation) and the flow injection method (consecutive analysis).
UR - http://www.scopus.com/inward/record.url?scp=85117270691&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85117270691&partnerID=8YFLogxK
U2 - 10.2116/analsci.21P009
DO - 10.2116/analsci.21P009
M3 - Article
C2 - 34629359
AN - SCOPUS:85117270691
VL - 37
SP - 1373
EP - 1377
JO - Analytical Sciences
JF - Analytical Sciences
SN - 0910-6340
IS - 10
ER -