Consensus and variations in cell line specificity among human metapneumovirus strains

Naganori Nao, Ko Sato, Junya Yamagishi, Maino Tahara, Yuichiro Nakatsu, Fumio Seki, Hiroshi Katoh, Aiko Ohnuma, Yuta Shirogane, Masahiro Hayashi, Tamio Suzuki, Hideaki Kikuta, Hidekazu Nishimura, Makoto Takeda

研究成果: ジャーナルへの寄稿記事

抄録

Human metapneumovirus (HMPV) has been a notable etiological agent of acute respiratory infection in humans, but it was not discovered until 2001, because HMPV replicates only in a limited number of cell lines and the cytopathic effect (CPE) is often mild. To promote the study of HMPV, several groups have generated green fluorescent protein (GFP)-expressing recombinant HMPV strains (HMPVGFP). However, the growing evidence has complicated the understanding of cell line specificity of HMPV, because it seems to vary notably among HMPV strains. In addition, unique A2b clade HMPV strains with a 180-nucleotide duplication in the G gene (HMPV A2b180nt-dup strains) have recently been detected. In this study, we reevaluated and compared the cell line specificity of clinical isolates of HMPV strains, including the novel HMPV A2b180nt-dup strains, and six recombinant HMPVGFP strains, including the newly generated recombinant HMPV A2b180nt-dup strain, MG0256-EGFP. Our data demonstrate that VeroE6 and LLC-MK2 cells generally showed the highest infectivity with any clinical isolates and recombinant HMPVGFP strains. Other human-derived cell lines (BEAS-2B, A549, HEK293, MNT-1, and HeLa cells) showed certain levels of infectivity with HMPV, but these were significantly lower than those of VeroE6 and LLC-MK2 cells. Also, the infectivity in these suboptimal cell lines varied greatly among HMPV strains. The variations were not directly related to HMPV genotypes, cell lines used for isolation and propagation, specific genome mutations, or nucleotide duplications in the G gene. Thus, these variations in suboptimal cell lines are likely intrinsic to particular HMPV strains. Progress of Research on Infectious Disease for Global Endemic (J-PRIDE and JP18fm0208005) and the Advanced Research and Development for Medical Innovation (AMED-CREST and JP18gm0910005) to M.Takeda.

元の言語英語
記事番号e0215822
ジャーナルPloS one
14
発行部数4
DOI
出版物ステータス出版済み - 4 1 2019

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Human metapneumovirus
Metapneumovirus
Cells
cell lines
Cell Line
Genes
pathogenicity
Nucleotides
nucleotides
Green Fluorescent Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Nao, N., Sato, K., Yamagishi, J., Tahara, M., Nakatsu, Y., Seki, F., ... Takeda, M. (2019). Consensus and variations in cell line specificity among human metapneumovirus strains. PloS one, 14(4), [e0215822]. https://doi.org/10.1371/journal.pone.0215822

Consensus and variations in cell line specificity among human metapneumovirus strains. / Nao, Naganori; Sato, Ko; Yamagishi, Junya; Tahara, Maino; Nakatsu, Yuichiro; Seki, Fumio; Katoh, Hiroshi; Ohnuma, Aiko; Shirogane, Yuta; Hayashi, Masahiro; Suzuki, Tamio; Kikuta, Hideaki; Nishimura, Hidekazu; Takeda, Makoto.

:: PloS one, 巻 14, 番号 4, e0215822, 01.04.2019.

研究成果: ジャーナルへの寄稿記事

Nao, N, Sato, K, Yamagishi, J, Tahara, M, Nakatsu, Y, Seki, F, Katoh, H, Ohnuma, A, Shirogane, Y, Hayashi, M, Suzuki, T, Kikuta, H, Nishimura, H & Takeda, M 2019, 'Consensus and variations in cell line specificity among human metapneumovirus strains', PloS one, 巻. 14, 番号 4, e0215822. https://doi.org/10.1371/journal.pone.0215822
Nao N, Sato K, Yamagishi J, Tahara M, Nakatsu Y, Seki F その他. Consensus and variations in cell line specificity among human metapneumovirus strains. PloS one. 2019 4 1;14(4). e0215822. https://doi.org/10.1371/journal.pone.0215822
Nao, Naganori ; Sato, Ko ; Yamagishi, Junya ; Tahara, Maino ; Nakatsu, Yuichiro ; Seki, Fumio ; Katoh, Hiroshi ; Ohnuma, Aiko ; Shirogane, Yuta ; Hayashi, Masahiro ; Suzuki, Tamio ; Kikuta, Hideaki ; Nishimura, Hidekazu ; Takeda, Makoto. / Consensus and variations in cell line specificity among human metapneumovirus strains. :: PloS one. 2019 ; 巻 14, 番号 4.
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