Constitutive expression of phosphoketolase, a key enzyme for metabolic shift from homo- to heterolactic fermentation in Enterococcus mundtii QU 25

Keisuke Nabeta, Satoru Watanabe, Taku Chibazakura, Takeshi Zendo, Kenji Sonomoto, Mariko Shimizu-Kadota, Hirofumi Yoshikawa

研究成果: ジャーナルへの寄稿コメント/討論

抄録

Phosphoketolase (PK) is responsible for heterolactic fermentation; however, the PK gene of Enterococcus mundtii QU 25, xfpA, is transcribed constitutively, even under homolactic fermentation conditions. In order to deduce the regulatory mechanisms of PK activity in QU 25, XfpA levels in QU 25 cells under hetero- and homolactic fermentation conditions were tested using western blotting. The results showed that the XfpA protein expression was similar under both conditions and that the expression products formed complexes, most likely homodimers, indicating that the regulation of PK activity is downstream of translation.

元の言語英語
ページ(範囲)111-114
ページ数4
ジャーナルBioscience of Microbiota, Food and Health
38
発行部数3
DOI
出版物ステータス出版済み - 1 1 2019

Fingerprint

phosphoketolase
Enterococcus mundtii
Enterococcus
Homo
Fermentation
fermentation
Enzymes
enzymes
translation (genetics)
Western blotting
protein synthesis
Western Blotting
Genes

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Gastroenterology

これを引用

Constitutive expression of phosphoketolase, a key enzyme for metabolic shift from homo- to heterolactic fermentation in Enterococcus mundtii QU 25. / Nabeta, Keisuke; Watanabe, Satoru; Chibazakura, Taku; Zendo, Takeshi; Sonomoto, Kenji; Shimizu-Kadota, Mariko; Yoshikawa, Hirofumi.

:: Bioscience of Microbiota, Food and Health, 巻 38, 番号 3, 01.01.2019, p. 111-114.

研究成果: ジャーナルへの寄稿コメント/討論

Nabeta, Keisuke ; Watanabe, Satoru ; Chibazakura, Taku ; Zendo, Takeshi ; Sonomoto, Kenji ; Shimizu-Kadota, Mariko ; Yoshikawa, Hirofumi. / Constitutive expression of phosphoketolase, a key enzyme for metabolic shift from homo- to heterolactic fermentation in Enterococcus mundtii QU 25. :: Bioscience of Microbiota, Food and Health. 2019 ; 巻 38, 番号 3. pp. 111-114.
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abstract = "Phosphoketolase (PK) is responsible for heterolactic fermentation; however, the PK gene of Enterococcus mundtii QU 25, xfpA, is transcribed constitutively, even under homolactic fermentation conditions. In order to deduce the regulatory mechanisms of PK activity in QU 25, XfpA levels in QU 25 cells under hetero- and homolactic fermentation conditions were tested using western blotting. The results showed that the XfpA protein expression was similar under both conditions and that the expression products formed complexes, most likely homodimers, indicating that the regulation of PK activity is downstream of translation.",
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AU - Nabeta, Keisuke

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AU - Chibazakura, Taku

AU - Zendo, Takeshi

AU - Sonomoto, Kenji

AU - Shimizu-Kadota, Mariko

AU - Yoshikawa, Hirofumi

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