Construction and application of a functional library of cytochrome p450 monooxygenases from the filamentous fungus Aspergillus oryzae

K. H.M. Nazmul Hussain Nazir, Hirofumi Ichinose, Hiroyuki Wariishi

研究成果: ジャーナルへの寄稿記事

22 引用 (Scopus)

抄録

A functional library of cytochrome P450 monooxygenases from Aspergillus oryzae (AoCYPs) was constructed in which 121 isoforms were coexpressed with yeast NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae. Using this functional library, novel catalytic functions of AoCYPs, such as catalytic potentials of CYP57B3 against genistein, were elucidated for the first time. Comprehensive functional screening promises rapid characterization of catalytic potentials and utility of AoCYPs.

元の言語英語
ページ(範囲)3147-3150
ページ数4
ジャーナルApplied and environmental microbiology
77
発行部数9
DOI
出版物ステータス出版済み - 5 1 2011

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Aspergillus oryzae
NADPH-Ferrihemoprotein Reductase
Genistein
Mixed Function Oxygenases
cytochrome P-450
Cytochrome P-450 Enzyme System
Libraries
cytochrome
Saccharomyces cerevisiae
Protein Isoforms
Fungi
Yeasts
fungus
fungi
genistein
oxidoreductases
NADP (coenzyme)
yeast
yeasts
screening

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

これを引用

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AU - Wariishi, Hiroyuki

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