Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter

Miyako Udono, Tsukasa Fujiki, Makiko Yamashita, Kazuyuki Katsuki, Masaaki Funata, Yusuke Imada, Kaichi Yoshizaki, Sanetaka Shirahata, Yoshinori Katakura

研究成果: ジャーナルへの寄稿記事

抄録

We constructed a novel cancer-specific regulatable adenoviral expression system comprising two vectors: one expressing rtTA, a reverse tetracycline transactivator regulated by the human telomerase reverse transcriptase (hTERT) gene promoter, the other expressing the target gene regulated by the tetracycline response element (TRE). rtTA transactivates target gene expression in the presence of doxycycline. Using these vectors, we constructed an adenoviral expression system, the Tel-On system, which enables highly efficient target gene transduction. This system enabled efficient and regulatable cancer-specific gene expression, and can be used in targeted cancer gene therapy.

元の言語英語
ページ(範囲)1638-1641
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
72
発行部数6
DOI
出版物ステータス出版済み - 7 1 2008

Fingerprint

telomerase
RNA-directed DNA polymerase
Genes
Neoplasm Genes
promoter regions
Tetracycline
tetracycline
Gene expression
neoplasms
Gene Expression
Gene therapy
Neoplasms
gene expression
doxycycline
Trans-Activators
gene therapy
genes
Doxycycline
response elements
Response Elements

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

これを引用

Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter. / Udono, Miyako; Fujiki, Tsukasa; Yamashita, Makiko; Katsuki, Kazuyuki; Funata, Masaaki; Imada, Yusuke; Yoshizaki, Kaichi; Shirahata, Sanetaka; Katakura, Yoshinori.

:: Bioscience, Biotechnology and Biochemistry, 巻 72, 番号 6, 01.07.2008, p. 1638-1641.

研究成果: ジャーナルへの寄稿記事

Udono, Miyako ; Fujiki, Tsukasa ; Yamashita, Makiko ; Katsuki, Kazuyuki ; Funata, Masaaki ; Imada, Yusuke ; Yoshizaki, Kaichi ; Shirahata, Sanetaka ; Katakura, Yoshinori. / Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter. :: Bioscience, Biotechnology and Biochemistry. 2008 ; 巻 72, 番号 6. pp. 1638-1641.
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AU - Funata, Masaaki

AU - Imada, Yusuke

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