抄録
We constructed a novel cancer-specific regulatable adenoviral expression system comprising two vectors: one expressing rtTA, a reverse tetracycline transactivator regulated by the human telomerase reverse transcriptase (hTERT) gene promoter, the other expressing the target gene regulated by the tetracycline response element (TRE). rtTA transactivates target gene expression in the presence of doxycycline. Using these vectors, we constructed an adenoviral expression system, the Tel-On system, which enables highly efficient target gene transduction. This system enabled efficient and regulatable cancer-specific gene expression, and can be used in targeted cancer gene therapy.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 1638-1641 |
| ページ数 | 4 |
| ジャーナル | Bioscience, Biotechnology and Biochemistry |
| 巻 | 72 |
| 発行部数 | 6 |
| DOI | |
| 出版物ステータス | 出版済み - 7 1 2008 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
- Biotechnology
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Chemistry (miscellaneous)
- Applied Microbiology and Biotechnology
- Food Science
これを引用
Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter. / Udono, Miyako; Fujiki, Tsukasa; Yamashita, Makiko; Katsuki, Kazuyuki; Funata, Masaaki; Imada, Yusuke; Yoshizaki, Kaichi; Shirahata, Sanetaka; Katakura, Yoshinori.
：: Bioscience, Biotechnology and Biochemistry, 巻 72, 番号 6, 01.07.2008, p. 1638-1641.研究成果: ジャーナルへの寄稿 › 記事
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TY - JOUR
T1 - Construction of a regulatable cancer-specific adenoviral expression system using human telomerase reverse transcriptase gene promoter
AU - Udono, Miyako
AU - Fujiki, Tsukasa
AU - Yamashita, Makiko
AU - Katsuki, Kazuyuki
AU - Funata, Masaaki
AU - Imada, Yusuke
AU - Yoshizaki, Kaichi
AU - Shirahata, Sanetaka
AU - Katakura, Yoshinori
PY - 2008/7/1
Y1 - 2008/7/1
N2 - We constructed a novel cancer-specific regulatable adenoviral expression system comprising two vectors: one expressing rtTA, a reverse tetracycline transactivator regulated by the human telomerase reverse transcriptase (hTERT) gene promoter, the other expressing the target gene regulated by the tetracycline response element (TRE). rtTA transactivates target gene expression in the presence of doxycycline. Using these vectors, we constructed an adenoviral expression system, the Tel-On system, which enables highly efficient target gene transduction. This system enabled efficient and regulatable cancer-specific gene expression, and can be used in targeted cancer gene therapy.
AB - We constructed a novel cancer-specific regulatable adenoviral expression system comprising two vectors: one expressing rtTA, a reverse tetracycline transactivator regulated by the human telomerase reverse transcriptase (hTERT) gene promoter, the other expressing the target gene regulated by the tetracycline response element (TRE). rtTA transactivates target gene expression in the presence of doxycycline. Using these vectors, we constructed an adenoviral expression system, the Tel-On system, which enables highly efficient target gene transduction. This system enabled efficient and regulatable cancer-specific gene expression, and can be used in targeted cancer gene therapy.
UR - http://www.scopus.com/inward/record.url?scp=45849131979&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=45849131979&partnerID=8YFLogxK
U2 - 10.1271/bbb.70587
DO - 10.1271/bbb.70587
M3 - Article
C2 - 18540079
AN - SCOPUS:45849131979
VL - 72
SP - 1638
EP - 1641
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 6
ER -