Construction of a series of pACYC-derived plasmid vectors

Yoshio Nakano, Yasuo Yoshida, Yoshihisa Yamashita, Toshihiko Koga

研究成果: ジャーナルへの寄稿記事

98 引用 (Scopus)

抄録

A series of cloning vectors has been constructed from pACYC177 and pACYC184. These vectors contain a MCS, the lacZα reporter gene, and P15A ori. Plasmids pMBL18, pMBL19 and pMBLcos express the bla gene encoding ampicillin resistance, while pMCL200 and pMCL210 contain the cat gene encoding chloramphenicol resistance. In addition, the cosmid pMBLcos was constructed with the P15A ori. These vectors are useful for cloning or subcloning genes which are unstable and/or toxic in high-copy-number vectors.

元の言語英語
ページ(範囲)157-158
ページ数2
ジャーナルGene
162
発行部数1
DOI
出版物ステータス出版済み - 8 30 1995

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Genetic Vectors
Plasmids
Chloramphenicol Resistance
Genes
Ampicillin Resistance
Cosmids
Lac Operon
Poisons
Reporter Genes
Cats

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Construction of a series of pACYC-derived plasmid vectors. / Nakano, Yoshio; Yoshida, Yasuo; Yamashita, Yoshihisa; Koga, Toshihiko.

:: Gene, 巻 162, 番号 1, 30.08.1995, p. 157-158.

研究成果: ジャーナルへの寄稿記事

Nakano, Yoshio ; Yoshida, Yasuo ; Yamashita, Yoshihisa ; Koga, Toshihiko. / Construction of a series of pACYC-derived plasmid vectors. :: Gene. 1995 ; 巻 162, 番号 1. pp. 157-158.
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