Construction of piggyBac-based vectors using visible and drug-resistance marker for introducing foreign genes into silkworm cultured cells

Tsuneyuki Tatsuke, Sun Mee Hong, Hiromi Tobata, Hiroaki Mon, Jae Man Lee, Yutaka Kawaguchi, Takahiro Kusakabe

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

Transgenic organisms have been indispensable for modern genetic analysis, such as over expression or knocked-down of the genes of interest. Transposon is one of the most efficient tools for introducing foreign DNA sequences into host genome. DNA transposon, such as piggyBac, Hermes, Minos, hobo, and mariner, have been identified in insects and have been used successfully as vectors for germline tarsnformation in various insect species. piggyBac-based transformation vectors have been broadly used in generating transgenic silkworm. However, there are few studies reporting vectors for transformation of cultured B. mori cells. In this study, we constructed new piggyBac-based vectors pPigGate, which have visible and drug selectable marker, PuroDsRed or GFPZeo in cultured cells. In order to access the utility of these vectors, we introduced BmHop2 and BmMnd1, which are meiosis specific recombination proteins, into cultured B. mori cells using the pPigGate.

本文言語英語
ページ(範囲)397-400
ページ数4
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
54
2
出版ステータス出版済み - 10 1 2009

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 農業および作物学

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