Construction of several deletion mutants for genes involved in biofilm formation and recovery of heat-injured Salmonella: ΔagfA and ΔbcsA mutants of Salmonella enteritidis; ΔahpC, ΔahpF, and ΔkatG mutants of S. Typhimurium; and ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium

Ken-ichi Honjoh, Yoshikazu Hashimoto, Satoshi Shimotsu, Hsu Ming Wen, Madoka Kiriki, Kimitaka Naito, Mio Tokugawa, Emiko Satake, Hiroshi Kobayashi, Takahisa Miyamoto

研究成果: ジャーナルへの寄稿学術誌査読

3 被引用数 (Scopus)

抄録

Salmonella is one of major food-poisoning bacteria. In food companies, controlling the bacterial growth is critically important to continue provision of good products to customers.. At first, to study the attachment of the bacteria to food materials and final products, we focused on biofilm formation that is thought to be one of the reasons of the attachment. Thus, the genes related in formation of biofilm were also focused on, and ΔagfA and ΔbcsA mutants of Salmonella Enteritidis were constructed by using 2-step PCR and pKOBEGA helper plasmid. Secondary, we focused on heat-injured Salmonella after sublethal heat treatment as previously reported (Kobayashi et al., 2005). The heat-injured Salmonella showed expression of several specific genes to recover the bacterial functional state as well as other bacteria. To clarify the mechanism of the recovery, several gene mutants (ΔahpC, ΔahpF, and ΔkatG mutants of Salmonella Typhimurium, and of ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium) were constructed.

本文言語英語
ページ(範囲)421-431
ページ数11
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
54
2
出版ステータス出版済み - 10月 2009

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 農業および作物学

フィンガープリント

「Construction of several deletion mutants for genes involved in biofilm formation and recovery of heat-injured Salmonella: ΔagfA and ΔbcsA mutants of Salmonella enteritidis; ΔahpC, ΔahpF, and ΔkatG mutants of S. Typhimurium; and ΔrpoE, ΔrpoH, and ΔrpoS mutants of S. Enteritidis and S. Typhimurium」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル