Construction of transgenic ipomoea obscura that exhibits new reddish leaf and flower colors due to introduction of β-carotene ketolase and hydroxylase genes

Motoyasu Otani, Kosuke Kitayama, Hiroki Ishikuro, Jun Ichiro Hattan, Takashi Maoka, Hisashi Harada, Yuko Shiotani, Akane Eguchi, Eiji Nitasaka, Norihiko Misawa

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

Ipomoea obscura, small white morning glory, is an ornamental plant belonging to the family Convolvulaceae, and cultivated worldwide. I. obscura generates white petals including a pale-yellow colored star-shaped center (flower vein). Its fully opened flowers were known to accumulate trace amounts of carotenoids such as β-carotene. In the present study, the embryogenic calli of I. obscura, were successfully produced through its immature embryo culture, and co-cultured with Agrobacterium tumefaciens carrying the β-carotene 4,4′-ketolase (crtW) and β-carotene 3,3′-hydroxylase (crtZ) genes for astaxanthin biosynthesis in addition to the isopentenyl diphosphate isomerase (idi) and hygromycin resistance genes. Transgenic plants, in which these four genes were introduced, were regenerated from the infected calli. They generated bronze (reddish green) leaves and novel petals that exhibited a color change from pale-yellow to pale-orange in the star-shaped center part. Especially, the color of their withered leaves changed drastically. HPLC-PDA-MS analysis showed that the expanded leaves of a transgenic line (T0) produced astaxanthin (5.2% of total carotenoids), adonirubin (3.9%), canthaxanthin (3.8%), and 3-hydroxyechinenone (3.6%), which indicated that these ketocarotenoids corresponded to 16.5% of the total carotenoids produced there (530 µg g−1 fresh weight). Furthermore, the altered traits of the transgenic plants were found to be inherited to their progenies by self-crossing.

本文言語英語
ページ(範囲)219-226
ページ数8
ジャーナルPlant Biotechnology
38
2
DOI
出版ステータス出版済み - 2021

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 農業および作物学
  • 植物科学

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