Continuous cell culture monitoring using a compact microplate reader with a silicone optical technology-based spatial filter

Y. Nakashima, M. Kounoura, C. Malasuk, K. Nakakubo, N. Watanabe, S. Iwata, K. Morita, Yuji Oki, S. Kuhara, Kosuke Tashiro, Y. Nakanishi

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Continuous cell monitoring is very important for the maintenance and control of cell multiplication and differentiation. This paper presents a compact microplate reader that is able to continuously measure a 24-well microplate (6 × 4 wells) using the optical absorption measurement method. The 24-channel plate reader consisted of a spatial filter, light emitting diode light source, and color sensors and was similarly sized with the cell culture microwell plates. A spatial filter was previously fabricated by our group using silicone optical technology (SOT). This SOT-based spatial filter has an excellent noise reduction effect. Light reflection at the optical path interface can be absorbed and only forward light can be transmitted; accordingly, a larger S/N ratio than that of conventional optical systems is expected. The fabricated 24-channel plate reader permits real-time cell monitoring during cultivation on the clean bench and in cell culture conditions by incorporating the SOT spatial filter. Using the device, it was possible to continuously evaluate the concentration and pH of reagents in the 24 wells in real time. Moreover, cell activity and protein production were detectable using the device. These results suggest that the newly fabricated device is a promising tool for the evaluation of cell behaviors for cell management.

元の言語英語
記事番号035106
ジャーナルReview of Scientific Instruments
90
発行部数3
DOI
出版物ステータス出版済み - 3 1 2019

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Continuous cell culture
silicones
readers
Silicones
Cell culture
filters
Monitoring
cells
Light reflection
Noise abatement
Optical systems
Light absorption
Light emitting diodes
Light sources
Color
Proteins
Sensors
optical paths
noise reduction
multiplication

All Science Journal Classification (ASJC) codes

  • Instrumentation

これを引用

Nakashima, Y., Kounoura, M., Malasuk, C., Nakakubo, K., Watanabe, N., Iwata, S., ... Nakanishi, Y. (2019). Continuous cell culture monitoring using a compact microplate reader with a silicone optical technology-based spatial filter. Review of Scientific Instruments, 90(3), [035106]. https://doi.org/10.1063/1.5054824

Continuous cell culture monitoring using a compact microplate reader with a silicone optical technology-based spatial filter. / Nakashima, Y.; Kounoura, M.; Malasuk, C.; Nakakubo, K.; Watanabe, N.; Iwata, S.; Morita, K.; Oki, Yuji; Kuhara, S.; Tashiro, Kosuke; Nakanishi, Y.

:: Review of Scientific Instruments, 巻 90, 番号 3, 035106, 01.03.2019.

研究成果: ジャーナルへの寄稿記事

Nakashima, Y, Kounoura, M, Malasuk, C, Nakakubo, K, Watanabe, N, Iwata, S, Morita, K, Oki, Y, Kuhara, S, Tashiro, K & Nakanishi, Y 2019, 'Continuous cell culture monitoring using a compact microplate reader with a silicone optical technology-based spatial filter', Review of Scientific Instruments, 巻. 90, 番号 3, 035106. https://doi.org/10.1063/1.5054824
Nakashima, Y. ; Kounoura, M. ; Malasuk, C. ; Nakakubo, K. ; Watanabe, N. ; Iwata, S. ; Morita, K. ; Oki, Yuji ; Kuhara, S. ; Tashiro, Kosuke ; Nakanishi, Y. / Continuous cell culture monitoring using a compact microplate reader with a silicone optical technology-based spatial filter. :: Review of Scientific Instruments. 2019 ; 巻 90, 番号 3.
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