Continuous Mechanical Indexing of Single-Cell Spheroids Using a Robot-Integrated Microfluidic Chip

Shinya Sakuma, Ko Nakahara, Fumihito Arai

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

In single-cell/spheroid analyses, flow cytometry plays a key role in high-throughput measurements. Among the considerable numbers of indexes available for a target evaluation, mechanical characteristics such as a Young's modulus have been focused upon as new indexes related to physiological condition. However, a continuous mechanical indexing system has yet to be achieved due to the difficulty in flow control, which brings about a fluctuation of the force sensor probe. In this letter, we propose an automated mechanical indexing system of spheroids. By utilizing a combination of two syringe pumps, we succeeded in positioning the target spheroids without an undesirable pinching. We conducted experiments on the continuous mechanical indexing of single-cell spheroids using 26 mesenchymal stem cell spheroids. The evaluation results of positioning showed that the error in the position control was 14.1 mum with a standard deviation of 87.7 mum, which corresponded to a measurement error of the Young's modulus of under 2%. In addition, we succeeded in achieving a measurement throughput of 3.14 spheroids per minute, which is approximately 2 times faster than a manual operation. Finally, we demonstrated the on-chip sorting of single spheroids based on the mechanical index to show the adaptability of the proposed method toward the flow cytometry.

本文言語英語
論文番号8740974
ページ(範囲)2973-2980
ページ数8
ジャーナルIEEE Robotics and Automation Letters
4
3
DOI
出版ステータス出版済み - 7 2019
外部発表はい

All Science Journal Classification (ASJC) codes

  • 制御およびシステム工学
  • 生体医工学
  • 人間とコンピュータの相互作用
  • 機械工学
  • コンピュータ ビジョンおよびパターン認識
  • コンピュータ サイエンスの応用
  • 制御と最適化
  • 人工知能

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