Continuous production of human erythropoietin by immobilized recombinant L-929 cells

Shuji Terashima, Masamichi Kamihira, Tatsuya Ogawa, Masanao Ohno, Shinji Iijima, Takeshi Kobayashi

研究成果: ジャーナルへの寄稿学術誌査読

16 被引用数 (Scopus)

抄録

Recombinant L-929 cells transfected with the human erythropoietin (EPO) gene were immobilized in a macroporous cellulosic support and its derivatives in which charged groups or cell attachment factors were introduced. The immobilized cells were cultured in serum-containing and serum-free media. Comparable production of EPO was observed even in the serum-free medium when a support modified by polyethyleneimine was used for immobilization. The cells immobilized on the supports were cultured in fluidized-bed and inner-loop type air-lift bioreactors for continuous production of EPO. A high cell density of more than 2 × 107 cells/cm3-support and high EPO productivity were achieved and maintained for 50 d through the use of the inner-loop type air-lift bioreactor. The productivity was 13.4-fold higher than that of conventional static cultures in petri-dishes.

本文言語英語
ページ(範囲)52-56
ページ数5
ジャーナルJournal of Fermentation and Bioengineering
77
1
DOI
出版ステータス出版済み - 1994
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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