Contraction of gut smooth muscle cells assessed by fluorescence imaging

Yohei Tokita, Hirotada Akiho, Kazuhiko Nakamura, Eikichi Ihara, Masahiro Yamamoto

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Here we discuss the development of a novel cell imaging system for the evaluation of smooth muscle cell (SMC) contraction. SMCs were isolated from the circular and longitudinal muscular layers of mouse small intestine by enzymatic digestion. SMCs were stimulated by test agents, thereafter fixed in acrolein. Actin in fixed SMCs was stained with phalloidin and cell length was determined by measuring diameter at the large end of phalloidin-stained strings within the cells. The contractile response was taken as the decrease in the average length of a population of stimulated-SMCs. Various mediators and chemically identified compounds of daikenchuto (DKT), pharmaceutical-grade traditional Japanese prokinetics, were examined. Verification of the integrity of SMC morphology by phalloidin and DAPI staining and semi-automatic measurement of cell length using an imaging analyzer was a reliable method by which to quantify the contractile response. Serotonin, substance P, prostaglandin E2 and histamine induced SMC contraction in concentration-dependent manner. Two components of DKT, hydroxy-α-sanshool and hydroxy-β-sanshool, induced contraction of SMCs. We established a novel cell imaging technique to evaluate SMC contractility. This method may facilitate investigation into SMC activity and its role in gastrointestinal motility, and may assist in the discovery of new prokinetic agents.

元の言語英語
ページ(範囲)344-351
ページ数8
ジャーナルJournal of Pharmacological Sciences
127
発行部数3
DOI
出版物ステータス出版済み - 1 1 2015

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Optical Imaging
Smooth Muscle Myocytes
Phalloidine
Muscle Contraction
Acrolein
Gastrointestinal Motility
Substance P
Dinoprostone
Histamine
Small Intestine
Actins
Digestion
Serotonin
Staining and Labeling
Pharmaceutical Preparations
Population
sanshool
dai-kenchu-to

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

これを引用

Contraction of gut smooth muscle cells assessed by fluorescence imaging. / Tokita, Yohei; Akiho, Hirotada; Nakamura, Kazuhiko; Ihara, Eikichi; Yamamoto, Masahiro.

:: Journal of Pharmacological Sciences, 巻 127, 番号 3, 01.01.2015, p. 344-351.

研究成果: ジャーナルへの寄稿記事

Tokita, Yohei ; Akiho, Hirotada ; Nakamura, Kazuhiko ; Ihara, Eikichi ; Yamamoto, Masahiro. / Contraction of gut smooth muscle cells assessed by fluorescence imaging. :: Journal of Pharmacological Sciences. 2015 ; 巻 127, 番号 3. pp. 344-351.
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