TY - JOUR
T1 - Contrast-tuneable microscopy for single-shot real-time imaging
AU - Shimizu, Isao
AU - Saikawa, Yoshinori
AU - Uno, Katsuhiro
AU - Kano, Hideaki
AU - Shimizu, Seishi
N1 - Funding Information:
We are grateful to Professor Masayuki Noguchi (Faculty of Medicine, University of Tsukuba) for providing us lung cancer cell samples (imaged in Fig. 7) and for his helpful advice, and to Dr Naoki Fukutake (Nikon) for useful discussion. I.S. gratefully acknowledges the supports from the Ibaraki Global Niche Top Companies Incubation Project and the Ichimura Foundation of New Technology.
Publisher Copyright:
© 2020 EDP Sciences.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - A novel real image in-line laser holography has enabled a tuneable image contrast, edge sharpness, and visualization of sub-wavelength structures, using a simple pair of filters and large-diameter lenses that can incorporate higher-order scattered beams. Demonstrated also are the accuracy in object sizing and the ease of imaging along the focal depth, based on a single-shot imaging via holographic principle. In addition, the use of broad, collimated laser beam for irradiation has led to a wider field of view, making it particularly useful for an extensive monitoring of, and sweeping search for, cells and microbial colonies and for the real-time imaging of cancer-cell dynamics.
AB - A novel real image in-line laser holography has enabled a tuneable image contrast, edge sharpness, and visualization of sub-wavelength structures, using a simple pair of filters and large-diameter lenses that can incorporate higher-order scattered beams. Demonstrated also are the accuracy in object sizing and the ease of imaging along the focal depth, based on a single-shot imaging via holographic principle. In addition, the use of broad, collimated laser beam for irradiation has led to a wider field of view, making it particularly useful for an extensive monitoring of, and sweeping search for, cells and microbial colonies and for the real-time imaging of cancer-cell dynamics.
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U2 - 10.1051/epjap/2020200101
DO - 10.1051/epjap/2020200101
M3 - Article
AN - SCOPUS:85092055786
VL - 91
JO - Journal De Physique, III
JF - Journal De Physique, III
SN - 1286-0042
IS - 3
M1 - 30701
ER -
