Contrast-tuneable microscopy for single-shot real-time imaging

Isao Shimizu; Yoshinori Saikawa; Katsuhiro Uno; Hideaki Kano; Seishi Shimizu

doi:10.1051/epjap/2020200101

Contrast-tuneable microscopy for single-shot real-time imaging

Isao Shimizu, Yoshinori Saikawa, Katsuhiro Uno, Hideaki Kano, Seishi Shimizu

研究成果: Contribution to journal › Article › 査読

抄録

A novel real image in-line laser holography has enabled a tuneable image contrast, edge sharpness, and visualization of sub-wavelength structures, using a simple pair of filters and large-diameter lenses that can incorporate higher-order scattered beams. Demonstrated also are the accuracy in object sizing and the ease of imaging along the focal depth, based on a single-shot imaging via holographic principle. In addition, the use of broad, collimated laser beam for irradiation has led to a wider field of view, making it particularly useful for an extensive monitoring of, and sweeping search for, cells and microbial colonies and for the real-time imaging of cancer-cell dynamics.

本文言語	英語
論文番号	30701
ジャーナル	EPJ Applied Physics
巻	91
号	3
DOI	https://doi.org/10.1051/epjap/2020200101
出版ステータス	出版済み - 9 1 2020
外部発表	はい

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics

文献へのアクセス

10.1051/epjap/2020200101

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引用スタイル

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abstract = "A novel real image in-line laser holography has enabled a tuneable image contrast, edge sharpness, and visualization of sub-wavelength structures, using a simple pair of filters and large-diameter lenses that can incorporate higher-order scattered beams. Demonstrated also are the accuracy in object sizing and the ease of imaging along the focal depth, based on a single-shot imaging via holographic principle. In addition, the use of broad, collimated laser beam for irradiation has led to a wider field of view, making it particularly useful for an extensive monitoring of, and sweeping search for, cells and microbial colonies and for the real-time imaging of cancer-cell dynamics.",

author = "Isao Shimizu and Yoshinori Saikawa and Katsuhiro Uno and Hideaki Kano and Seishi Shimizu",

note = "Funding Information: We are grateful to Professor Masayuki Noguchi (Faculty of Medicine, University of Tsukuba) for providing us lung cancer cell samples (imaged in Fig. 7) and for his helpful advice, and to Dr Naoki Fukutake (Nikon) for useful discussion. I.S. gratefully acknowledges the supports from the Ibaraki Global Niche Top Companies Incubation Project and the Ichimura Foundation of New Technology.",

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AU - Shimizu, Seishi

N1 - Funding Information: We are grateful to Professor Masayuki Noguchi (Faculty of Medicine, University of Tsukuba) for providing us lung cancer cell samples (imaged in Fig. 7) and for his helpful advice, and to Dr Naoki Fukutake (Nikon) for useful discussion. I.S. gratefully acknowledges the supports from the Ibaraki Global Niche Top Companies Incubation Project and the Ichimura Foundation of New Technology.

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