Contribution of conformational stability of hen lysozyme to induction of type 2 T-helper immune responses

Takanori So, Hiro O. Ito, Masato Hirata, Tadashi Ueda, Taiji Imoto

研究成果: ジャーナルへの寄稿記事

30 引用 (Scopus)

抄録

It is important to identify characteristics that confer on proteins the potential to induce allergenic sensitization and allergenic disease. Protein allergens carry T-cell epitopes that are capable of inducing a type 2 T helper (Th2) cell response. There is limited information regarding factors that govern the allergenicity of proteins. We previously reported that a decrease in the conformational stability of hen-egg lysozyme (HEL) enhanced its capacity to activate HEL-specific T cells owing to the increased susceptibility to intracellular antigen processing. To determine whether the conformational stability of HEL makes for a critical contribution to allergenic sensitization in vivo, we immunized BALB/c mice with HEL derivatives of different conformational stability, but which retained a similar three-dimensional structure. The magnitude of in vivo T-cell responses, evaluated by ex vivo proliferative responses of lymph node T cells from mice primed with various HEL derivatives, was inversely correlated with conformational stability, as was interferon-γ (IFN-γ) and interleukin-4 (IL-4) production by splenic T cells in response to HEL. Immunization of the least stable derivative led to a potent IL-4 response and to immunoglobulin E (IgE) antibody production. We propose that the intrinsic allergenicity of proteins can be determined by the degree of conformational stability.

元の言語英語
ページ(範囲)259-268
ページ数10
ジャーナルImmunology
104
発行部数3
DOI
出版物ステータス出版済み - 11 26 2001

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Muramidase
T-Lymphocytes
Interleukin-4
Proteins
Th2 Cells
T-Lymphocyte Epitopes
Antigen Presentation
Helper-Inducer T-Lymphocytes
Allergens
Interferons
Immunoglobulin E
Antibody Formation
hen egg lysozyme
Immunization
Lymph Nodes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

これを引用

Contribution of conformational stability of hen lysozyme to induction of type 2 T-helper immune responses. / So, Takanori; Ito, Hiro O.; Hirata, Masato; Ueda, Tadashi; Imoto, Taiji.

:: Immunology, 巻 104, 番号 3, 26.11.2001, p. 259-268.

研究成果: ジャーナルへの寄稿記事

So, Takanori ; Ito, Hiro O. ; Hirata, Masato ; Ueda, Tadashi ; Imoto, Taiji. / Contribution of conformational stability of hen lysozyme to induction of type 2 T-helper immune responses. :: Immunology. 2001 ; 巻 104, 番号 3. pp. 259-268.
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