Contribution of maternal mRNA for maintenance of Ca2+-dependent reaggregating activity in dissociated cells of Xenopus laevis embryos

K. Shiokawa, Kosuke Tashiro, T. Oka, K. Yamana

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

Dissociated Xenopus laevis blastula cells, where reaggregation was inhibited in Ca2+-free medium, reaggregated immediately after the addition of Ca2+. This reaggregation was not inhibited by cordycepin or actinomycin D treatment during culture, although cycloheximide and puromycin were inhibitory. The reaggregation was not inhibited even when fertilized eggs were microinjected with cordycepin and their RNA synthesis was continuously inhibited through cleavage to blastula stages. In neurula cells, cordycepin treatment induced significant reduction in sizes of aggregates formed. These results suggest that the Ca2+-dependent reaggregating activity of blastula cells is maintained by the translation of maternal, rather than newly synthesized, mRNA.

元の言語英語
ページ(範囲)247-255
ページ数9
ジャーナルCell Differentiation
13
発行部数3
DOI
出版物ステータス出版済み - 1 1 1983

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Stored Messenger RNA
Blastula
Xenopus laevis
Embryonic Structures
Maintenance
Puromycin
Zygote
Dactinomycin
Cycloheximide
Mothers
RNA
Messenger RNA
cordycepin

All Science Journal Classification (ASJC) codes

  • Developmental Biology

これを引用

Contribution of maternal mRNA for maintenance of Ca2+-dependent reaggregating activity in dissociated cells of Xenopus laevis embryos. / Shiokawa, K.; Tashiro, Kosuke; Oka, T.; Yamana, K.

:: Cell Differentiation, 巻 13, 番号 3, 01.01.1983, p. 247-255.

研究成果: ジャーナルへの寄稿記事

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abstract = "Dissociated Xenopus laevis blastula cells, where reaggregation was inhibited in Ca2+-free medium, reaggregated immediately after the addition of Ca2+. This reaggregation was not inhibited by cordycepin or actinomycin D treatment during culture, although cycloheximide and puromycin were inhibitory. The reaggregation was not inhibited even when fertilized eggs were microinjected with cordycepin and their RNA synthesis was continuously inhibited through cleavage to blastula stages. In neurula cells, cordycepin treatment induced significant reduction in sizes of aggregates formed. These results suggest that the Ca2+-dependent reaggregating activity of blastula cells is maintained by the translation of maternal, rather than newly synthesized, mRNA.",
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AU - Yamana, K.

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