Control region and gastric specific transcription of the rat H+,K+-ATPase α subunit gene

Ko Ichi Oshiman, Kiyoto Motojima, Saleh Mahmood, Akira Shimada, Shigehiko Tamura, Masatomo Maeda, Masamitsu Futai

研究成果: Contribution to journalArticle

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The rat gastric H+,K+-ATPase α subunit gene was cloned and the nucleotide sequence of its 5′-upstream region was determined. Sequence comparison with the corresponding part of the human gene indicated the presence of highly conserved regions which may be important for specific transcription of the α subunit in gastric parietal cells. The amino-terminal sequence (Met-Gly-Lys-Ala-Glu-) of the rat enzyme was similar to those of the pig and human enzymes. The gene organization of the rat enzyme was also similar to that of the human gene: introns 1, 2 and 9 were located in exactly the same positions as those in the human gene, and, as in the latter, exon 6 was not separated by an intron. The sequences of introns 1 and 2 were highly conserved among the rat, human and pig genes, but were entirely different from those of Na+,K+-ATPase catalytic subunit genes. Northern blot hybridization indicated that the gene was transcribed only in gastric mucosa.

元の言語英語
ページ(範囲)250-254
ページ数5
ジャーナルFEBS Letters
281
発行部数1-2
DOI
出版物ステータス出版済み - 4 9 1991

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Oshiman, K. I., Motojima, K., Mahmood, S., Shimada, A., Tamura, S., Maeda, M., & Futai, M. (1991). Control region and gastric specific transcription of the rat H+,K+-ATPase α subunit gene. FEBS Letters, 281(1-2), 250-254. https://doi.org/10.1016/0014-5793(91)80404-Q