Controlled radical polymerization on β-sheet structures

Takeshi Mori, Shiro Yasutake, Keiji Minagawa, Masami Tanaka

研究成果: 書籍/レポート タイプへの寄稿会議への寄与


Solid-phase polymerization of diacetylene occurs when diacetylene monomer aligns with each other with a distance of 5 Å. In this study, we focused on the β-sheet of peptide to align the diacetylene because in that structure, peptide chain aligns with each other with a distance of 5 Å. Thus, we designed amino acid with diacetylene group on its side chain; Thr(DA). This amino acid was introduced in the center of the undecapeptide which was designed to form anti-parallel β-sheet. The peptide was synthesized based on the Boc-based solid-phase method. After finishing the elongation of the peptide chain, the peptide was cleaved from the resin by treatment with trifluoromethane sulfonic acid for one hour. In this process, the color of the solution changed from yellow to purple. The obtained purple-colored peptide was purified by reprecipitation using diethyl ether. The peptide had maximum absorption at 580 nm and the IR absorption resulting from unreacted diacetylene group (2260 cm -1) almost disappeared in the peptide. These results showed that the diacetylene group of peptide polymerized rapidly after cleaved from the resin. In the IR spectrum of the peptide, the absorptions resulting from both anti-parallel β-sheet (1632 and 1680 cm -1) and random coil (1662 cm -1) were observed. Because the polymerization proceeded so rapidly and completely, the polymerization would mainly occur in the anti-parallel β-sheet region of the peptide.

ホスト出版物のタイトル54th SPSJ Symposium on Macromolecules - Polymer Preprints, Japan
出版ステータス出版済み - 2005
イベント54th SPSJ Symposium on Macromolecules - Yamagata, 日本
継続期間: 9月 20 20059月 22 2005


その他54th SPSJ Symposium on Macromolecules

!!!All Science Journal Classification (ASJC) codes

  • 工学(全般)


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