Controlled release of PEG chain from gold nanorods: Targeted delivery to tumor

Takuro Niidome, Akira Ohga, Yasuyuki Akiyama, Kazuto Watanabe, Yasuro Niidome, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

35 引用 (Scopus)

抄録

Gold nanorods exhibit strong absorbance of light in the near infrared region, which penetrates deeply into tissues. Since the absorbed light energy is converted into heat, gold nanorods are expected to act as a contrast agent for in vivo bioimaging and as a thermal converter for photothermal therapy. To construct a gold nanorod targeted delivery system for tumor a peptide substrate for urokinase-type plasminogen activator (uPA), expressed specifically on malignant tumors, was inserted between the PEG chain and the surface of the gold nanorods. In other words, we constructed PEG-peptide-modified gold nanorods. After mixing the gold nanorods with uPA, the PEG chain was released from the surface of the gold and subsequently nanorod aggregation took place. The formation of the aggregation was monitored as a decrease in light absorption at 900 nm. Tumor homogenate induced a significant decrease in this absorption. Larger amount of the PEG-peptide-modified gold nanorods bound to cells expressing uPA in vitro compared with control gold nanorods, which had scrambled sequence of the peptide. The PEG-peptide-modified gold nanorods showed higher accumulation in tumor than the control after they were injected intravenously into tumor-bearing mice, however, the density of the peptide on the surface of the gold nanorods was a key factor of their biodistributions. This targeted delivery system, which responds to uPA activity, is expected to be a powerful tool for tumor bioimaging and photothermal tumor therapy.

元の言語英語
ページ(範囲)4453-4458
ページ数6
ジャーナルBioorganic and Medicinal Chemistry
18
発行部数12
DOI
出版物ステータス出版済み - 6 15 2010

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Nanotubes
Nanorods
Gold
Polyethylene glycols
Tumors
Neoplasms
Urokinase-Type Plasminogen Activator
Peptides
Bearings (structural)
Agglomeration
Hot Temperature
Light
Light absorption
Contrast Media
Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

これを引用

Controlled release of PEG chain from gold nanorods : Targeted delivery to tumor. / Niidome, Takuro; Ohga, Akira; Akiyama, Yasuyuki; Watanabe, Kazuto; Niidome, Yasuro; Mori, Takeshi; Katayama, Yoshiki.

:: Bioorganic and Medicinal Chemistry, 巻 18, 番号 12, 15.06.2010, p. 4453-4458.

研究成果: ジャーナルへの寄稿記事

Niidome, Takuro ; Ohga, Akira ; Akiyama, Yasuyuki ; Watanabe, Kazuto ; Niidome, Yasuro ; Mori, Takeshi ; Katayama, Yoshiki. / Controlled release of PEG chain from gold nanorods : Targeted delivery to tumor. :: Bioorganic and Medicinal Chemistry. 2010 ; 巻 18, 番号 12. pp. 4453-4458.
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