Coordinated leading and lagging strand DNA synthesis on a minicircular template

Joonsoo Lee, Paul D. Chastain, Takahiro Kusakabe, Jack D. Griffith, Charles C. Richardson

研究成果: Contribution to journalArticle査読

115 被引用数 (Scopus)


The coordinated synthesis of both leading and lagging DNA strands is thought to involve a dimeric DNA polymerase and a looping of the lagging strand so that both strands can be synthesized in the same direction. We have constructed a minicircle with a replication fork that permits an assessment of the stoichiometry of the proteins and a measurement of the synthesis of each strand. The replisome consisting of bacteriophage T7 DNA polymerase, helicase, primase, and single-stranded DNA-binding protein mediates coordinated replication. The criteria for coordination are fulfilled : (1) a replication loop is formed, (2) leading and lagging strand synthesis are coupled, (3) the lagging strand polymerase recycles from one Okazaki fragment to another, and (4) the length of Okazaki fragments is regulated. T7 single-stranded DNA-binding protein is essential for coordination.

ジャーナルMolecular Cell
出版ステータス出版済み - 6 1998

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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