Coronary Microvascular Endothelial Stunning after Acute Pressure Overload in the Conscious Dog Is Caused by Oxidant Processes: The Role of Angiotensin II Type I Receptor and NAD(P)H Oxidase

Shintaro Kinugawa, Heiner Post, Pawel M. Kaminski, Xiaoping Zhang, Xiaobin Xu, Harer Huang, Fabio A. Recchia, Manuel Ochoa, Michael S. Wolin, Gabor Kaley, Thomas H. Hintze

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Background-Few studies have examined the effect of acute pressure overload on endothelial function in the coronary microcirculation. Methods and Results-In instrumented conscious dogs with heart rate held constant, veratrine caused a cholinergic nitric oxide (NO)-dependent increase in coronary blood flow by 23±3 mL/min (Bezold-Jarisch reflex). Ten minutes after release of constriction of the ascending aorta to increase left ventricular (LV) systolic pressure to 214±5 mm Hg for 30 minutes, the veratrine-induced increase in coronary blood flow (7±1 mL/min) was reduced by 66% and remained depressed for 2 hours (ie, endothelial stunning [ES]). Nitrite production from isolated coronary microvessels during ES was not different from normal. Ascorbic acid (AA), losartan, or apocynin prevented ES. Myocardial oxygen consumption (MV̇O2) of LV tissue was measured in vitro in response to bradykinin with preincubation of angiotensin II for 30 minutes. Bradykinin (10-4 mol/L)-induced reduction in MV̇O2 was reversed in a concentration-dependent manner by angiotensin II (38±1% versus 19±2% at 10-8 mol/L) and restored by coincubation of AA (37±2%), tempol (33±2%), losartan (34±2%), or apocynin (36±1%). Exogenous NO-induced reduction in MV̇O2 was not altered by angiotensin II. Angiotensin II increased lucigenin-detectable superoxide anion in LV tissue in a manner that was inhibited by bradykinin, AA, tempol, losartan, or apocynin. Conclusions-Endothelial stunning is caused by oxidant processes inhibited by ascorbate, and the activation of NAD(P)H oxidase by increased angiotensin II plays an important role in this process.

元の言語英語
ページ(範囲)2934-2940
ページ数7
ジャーナルCirculation
108
発行部数23
DOI
出版物ステータス出版済み - 12 9 2003
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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