Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay

Akira Tsuchiya, Daisuke Asai, Jeong Hun Kang, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

To investigate the correlation between the counts per minute (CPM) by radioactivity assay and the phosphorylation ratio by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, we prepared 136 peptide substrates. The correlation coefficient of phosphorylation ratios to CPM was 0.77 for all samples. However, the more the numbers of positively charged amino acids increased, the more the correlation coefficient increased. Although positively charged amino acids can have an effect on the correlation results, MALDI-TOF MS analysis is a useful means for monitoring phosphorylated peptide and protein kinase activity instead of radioactivity assays.

本文言語英語
ページ(範囲)773-775
ページ数3
ジャーナルAnalytical Biochemistry
421
2
DOI
出版ステータス出版済み - 2 15 2012

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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