Coulometric detection of components in liquid plugs by microfabricated flow channel and electrode structures

Fumihiro Sassa, Hind Laghzali, Junji Fukuda, Hiroaki Suzuki

研究成果: Contribution to journalArticle査読

39 被引用数 (Scopus)


Coulometry has been demonstrated to be effective for determining the analyte in a liquid plug on the nanoliter-scale confined in a flow channel. A plug prepared in a rhombus structure of an auxiliary flow channel was placed on a thin-film three-electrode system, and hydrogen peroxide was detected as a model analyte. Under a fixed potential, the current decayed rapidly, particularly in shallow flow channels, thus making reproducible amperometric detection difficult. On the other hand, the increasing charge during coulometry facilitated the measurements. A constricted flow channel structure with an array of platinum strips for the working electrode was effective at efficiently consuming the analyte to improve the sensitivity and lower the detection limit. Compared to the case of a single short working electrode with the same area, a 4-fold increase in sensitivity was observed for the best combination of flow channel height and interstrip distance. With an increase in the generated current while maintaining the background at a low level, the detection limit was lowered from 1.3 μM to 410 nM using working electrodes with the same area. Furthermore, the processing of solutions containing l-glutamate or l-glutamate oxidase and the detection of l-glutamate were demonstrated.

ジャーナルAnalytical chemistry
出版ステータス出版済み - 10 15 2010

All Science Journal Classification (ASJC) codes

  • 分析化学


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