Coupling of STIM1 to store-operated Ca2+ entry through its constitutive and inducible movement in the endoplasmic reticulum

Yoshihiro Baba, Kenji Hayashi, Yoko Fujii, Akiko Mizushima, Hiroshi Watarai, Minoru Wakamori, Takuro Numaga, Yasuo Mori, Masamitsu Iino, Masaki Hikida, Tomohiro Kurosaki

研究成果: ジャーナルへの寄稿記事

245 引用 (Scopus)

抄録

Depletion of intracellular calcium (Ca2+) stores induces store-operated Ca2+ (SOC) entry across the plasma membrane (PM). STIM1, a putative Ca2+ sensor in the endoplasmic reticulum (ER), has been recently shown to be necessary for SOC channel activation. Here we show that STIM1 dynamically moves in tubulovesicular shape on the ER and its subcompartment in resting living cells, whereas, upon Ca2+ store depletion, it is rapidly redistributed into discrete puncta that are located underneath, but not inserted into the PM. Normal constitutive movement of STIM1 s mediated through the coiled-coil and Ser/Thr-rich C-terminal domains in the cytoplasmic region of STIM1, whereas subsequent inducible puncta formation further requires the sterile α motif domain protruding into the ER lumen. Each of these three domains (coiled-coil, Ser/Thr-rich, and sterile α motif) was essential for activating SOC channels. Hence, our findings based on structure-function experiments suggest that constitutive dynamic movement of STIM1 in the ER and its subcompartment is obligatory for subsequent depletion-dependent redistribution of STIM1 into puncta underneath the PM and activation of SOC channels.

元の言語英語
ページ(範囲)16704-16709
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
103
発行部数45
DOI
出版物ステータス出版済み - 11 7 2006

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Endoplasmic Reticulum
Cell Membrane
Calcium

All Science Journal Classification (ASJC) codes

  • General

これを引用

Coupling of STIM1 to store-operated Ca2+ entry through its constitutive and inducible movement in the endoplasmic reticulum. / Baba, Yoshihiro; Hayashi, Kenji; Fujii, Yoko; Mizushima, Akiko; Watarai, Hiroshi; Wakamori, Minoru; Numaga, Takuro; Mori, Yasuo; Iino, Masamitsu; Hikida, Masaki; Kurosaki, Tomohiro.

:: Proceedings of the National Academy of Sciences of the United States of America, 巻 103, 番号 45, 07.11.2006, p. 16704-16709.

研究成果: ジャーナルへの寄稿記事

Baba, Y, Hayashi, K, Fujii, Y, Mizushima, A, Watarai, H, Wakamori, M, Numaga, T, Mori, Y, Iino, M, Hikida, M & Kurosaki, T 2006, 'Coupling of STIM1 to store-operated Ca2+ entry through its constitutive and inducible movement in the endoplasmic reticulum', Proceedings of the National Academy of Sciences of the United States of America, 巻. 103, 番号 45, pp. 16704-16709. https://doi.org/10.1073/pnas.0608358103
Baba, Yoshihiro ; Hayashi, Kenji ; Fujii, Yoko ; Mizushima, Akiko ; Watarai, Hiroshi ; Wakamori, Minoru ; Numaga, Takuro ; Mori, Yasuo ; Iino, Masamitsu ; Hikida, Masaki ; Kurosaki, Tomohiro. / Coupling of STIM1 to store-operated Ca2+ entry through its constitutive and inducible movement in the endoplasmic reticulum. :: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; 巻 103, 番号 45. pp. 16704-16709.
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AU - Wakamori, Minoru

AU - Numaga, Takuro

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AU - Kurosaki, Tomohiro

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