Cross-linking versus non-cross-linking aggregation of gold nanoparticles induced by DNA hybridization: A comparison of the rapidity of solution color change

Guoqing Wang, Yoshitsugu Akiyama, Shota Shiraishi, Naoki Kanayama, Tohru Takarada, Mizuo Maeda

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

Gold nanoparticles densely modified with single-stranded DNA (ssDNA-AuNPs) form aggregates with cross-linker ssDNAs via duplex formation. Alternatively, the ssDNA-AuNPs are spontaneously aggregated at high ionic strength in a non-cross-linking manner when complementary ssDNAs are added to form fully matched duplexes. Both aggregation modes are accompanied by a red-to-purple color change, which has been exploited in various bioassays. The current study compares the rapidity of color change between the cross-linking and non-cross-linking aggregation modes under identical conditions. When a small number of cross-linker/complementary DNAs are provided, the cross-linking mode exhibited more rapid color change than the non-cross-linking mode. Conversely, with a large number of the DNAs, the non-cross-linking aggregation occurred more rapidly than the cross-linking counterpart. This finding allows one to select a more appropriate aggregation mode for application of ssDNA-AuNPs to colorimetric assays under given conditions.

元の言語英語
ページ(範囲)270-277
ページ数8
ジャーナルBioconjugate Chemistry
28
発行部数1
DOI
出版物ステータス出版済み - 1 1 2017

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Gold
Nanoparticles
DNA
Agglomeration
Color
Single-Stranded DNA
Biological Assay
Osmolar Concentration
Bioassay
Complementary DNA
Ionic strength
Assays

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

これを引用

Cross-linking versus non-cross-linking aggregation of gold nanoparticles induced by DNA hybridization : A comparison of the rapidity of solution color change. / Wang, Guoqing; Akiyama, Yoshitsugu; Shiraishi, Shota; Kanayama, Naoki; Takarada, Tohru; Maeda, Mizuo.

:: Bioconjugate Chemistry, 巻 28, 番号 1, 01.01.2017, p. 270-277.

研究成果: ジャーナルへの寄稿記事

Wang, Guoqing ; Akiyama, Yoshitsugu ; Shiraishi, Shota ; Kanayama, Naoki ; Takarada, Tohru ; Maeda, Mizuo. / Cross-linking versus non-cross-linking aggregation of gold nanoparticles induced by DNA hybridization : A comparison of the rapidity of solution color change. :: Bioconjugate Chemistry. 2017 ; 巻 28, 番号 1. pp. 270-277.
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