Cross-reactivity of various tetrahydrocannabinol metabolites with a monoclonal antibody against tetrahydrocannabinolic acid

K. Watanabe, M. Matsuda, Y. Tateoka, T. Kimura, T. Matsunaga, H. Tanaka, Y. Shoyama, I. Yamamoto

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

A monoclonal antibody (MAb-4A4) against Δ9-tetrahydrocannabinolic acid (THCA) was prepared and its cross-reactivity analyzed with various cannabinoids including tetrahydrocannabinol metabolites by competitive enzyme-linked immunoassay (ELISA). Δ9-THC (1-100 μg/ml) could be determined by the ELISA. Many metabolites of Δ8-THC and Δ9-THC reacted with the antibody, and their cross-reactivities were 44-157% of Δ8-THC. However, the antibody did not recognize the lipophilic compounds cholesterol, testosterone, β-carotene, androstene-3,17-dione or an endogenous cannabinoid, anandamide.

元の言語英語
ページ(範囲)310-313
ページ数4
ジャーナルJournal of Health Science
46
発行部数4
DOI
出版物ステータス出版済み - 1 1 2000

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Dronabinol
Metabolites
Monoclonal Antibodies
Acids
Cannabinoids
Immunoenzyme Techniques
Androstenes
Antibodies
Enzymes
Carotenoids
Testosterone
Cholesterol

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

これを引用

Watanabe, K., Matsuda, M., Tateoka, Y., Kimura, T., Matsunaga, T., Tanaka, H., ... Yamamoto, I. (2000). Cross-reactivity of various tetrahydrocannabinol metabolites with a monoclonal antibody against tetrahydrocannabinolic acid. Journal of Health Science, 46(4), 310-313. https://doi.org/10.1248/jhs.46.310

Cross-reactivity of various tetrahydrocannabinol metabolites with a monoclonal antibody against tetrahydrocannabinolic acid. / Watanabe, K.; Matsuda, M.; Tateoka, Y.; Kimura, T.; Matsunaga, T.; Tanaka, H.; Shoyama, Y.; Yamamoto, I.

:: Journal of Health Science, 巻 46, 番号 4, 01.01.2000, p. 310-313.

研究成果: ジャーナルへの寄稿記事

Watanabe, K, Matsuda, M, Tateoka, Y, Kimura, T, Matsunaga, T, Tanaka, H, Shoyama, Y & Yamamoto, I 2000, 'Cross-reactivity of various tetrahydrocannabinol metabolites with a monoclonal antibody against tetrahydrocannabinolic acid', Journal of Health Science, 巻. 46, 番号 4, pp. 310-313. https://doi.org/10.1248/jhs.46.310
Watanabe, K. ; Matsuda, M. ; Tateoka, Y. ; Kimura, T. ; Matsunaga, T. ; Tanaka, H. ; Shoyama, Y. ; Yamamoto, I. / Cross-reactivity of various tetrahydrocannabinol metabolites with a monoclonal antibody against tetrahydrocannabinolic acid. :: Journal of Health Science. 2000 ; 巻 46, 番号 4. pp. 310-313.
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AU - Watanabe, K.

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AU - Tanaka, H.

AU - Shoyama, Y.

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