Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy

Yasuhiro Utsumi, Yuzou Sano

研究成果: Chapter in Book/Report/Conference proceedingChapter

5 被引用数 (Scopus)

抄録

The protocol of cryoplaning techniques that to examine the distribution of water in living tree stems by cryoscanning electron microscopy have been developed and described. In brief, the procedures are as follows: First, a portion of transpiring stem is frozen in the standing state with liquid nitrogen to stabilize the water that is present in the conducting tissue. After filling with liquid nitrogen, discs are then collected from the frozen portion of the stem and stored in liquid nitrogen. The surface of disc is cleanly cut using a sliding microtome in a low temperature room at -20°C. Finally, the frozen sample is examined in a cryoscanning electron microscope after freeze-etching and metal coating.

本文言語英語
ホスト出版物のタイトルElectron Microscopy
ホスト出版物のサブタイトルMethods and Protocols
編集者John Kuo
ページ497-506
ページ数10
DOI
出版ステータス出版済み - 2 27 2007

出版物シリーズ

名前Methods in Molecular Biology
369
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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