抄録
The protocol of cryoplaning techniques that to examine the distribution of water in living tree stems by cryoscanning electron microscopy have been developed and described. In brief, the procedures are as follows: First, a portion of transpiring stem is frozen in the standing state with liquid nitrogen to stabilize the water that is present in the conducting tissue. After filling with liquid nitrogen, discs are then collected from the frozen portion of the stem and stored in liquid nitrogen. The surface of disc is cleanly cut using a sliding microtome in a low temperature room at -20°C. Finally, the frozen sample is examined in a cryoscanning electron microscope after freeze-etching and metal coating.
| 元の言語 | 英語 |
|---|---|
| ホスト出版物のタイトル | Electron Microscopy: Methods and Protocols |
| ページ | 497-506 |
| ページ数 | 10 |
| 巻 | 369 |
| DOI | |
| 出版物ステータス | 出版済み - 2 27 2007 |
出版物シリーズ
| 名前 | Methods in Molecular Biology |
|---|---|
| 巻 | 369 |
| ISSN（印刷物） | 10643745 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
これを引用
Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy. / Utsumi, Yasuhiro; Sano, Yuzou.
Electron Microscopy: Methods and Protocols. 巻 369 2007. p. 497-506 (Methods in Molecular Biology; 巻 369).研究成果: 著書/レポートタイプへの貢献 › 章
}
TY - CHAP
T1 - Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy
AU - Utsumi, Yasuhiro
AU - Sano, Yuzou
PY - 2007/2/27
Y1 - 2007/2/27
N2 - The protocol of cryoplaning techniques that to examine the distribution of water in living tree stems by cryoscanning electron microscopy have been developed and described. In brief, the procedures are as follows: First, a portion of transpiring stem is frozen in the standing state with liquid nitrogen to stabilize the water that is present in the conducting tissue. After filling with liquid nitrogen, discs are then collected from the frozen portion of the stem and stored in liquid nitrogen. The surface of disc is cleanly cut using a sliding microtome in a low temperature room at -20°C. Finally, the frozen sample is examined in a cryoscanning electron microscope after freeze-etching and metal coating.
AB - The protocol of cryoplaning techniques that to examine the distribution of water in living tree stems by cryoscanning electron microscopy have been developed and described. In brief, the procedures are as follows: First, a portion of transpiring stem is frozen in the standing state with liquid nitrogen to stabilize the water that is present in the conducting tissue. After filling with liquid nitrogen, discs are then collected from the frozen portion of the stem and stored in liquid nitrogen. The surface of disc is cleanly cut using a sliding microtome in a low temperature room at -20°C. Finally, the frozen sample is examined in a cryoscanning electron microscope after freeze-etching and metal coating.
UR - http://www.scopus.com/inward/record.url?scp=34548035630&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548035630&partnerID=8YFLogxK
U2 - 10.1385/1-59745-294-7:497
DO - 10.1385/1-59745-294-7:497
M3 - Chapter
C2 - 17656766
AN - SCOPUS:34548035630
SN - 1597452947
SN - 9781597452946
VL - 369
T3 - Methods in Molecular Biology
SP - 497
EP - 506
BT - Electron Microscopy: Methods and Protocols
ER -