Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy

Yasuhiro Utsumi, Yuzou Sano

研究成果: 著書/レポートタイプへの貢献

5 引用 (Scopus)

抄録

The protocol of cryoplaning techniques that to examine the distribution of water in living tree stems by cryoscanning electron microscopy have been developed and described. In brief, the procedures are as follows: First, a portion of transpiring stem is frozen in the standing state with liquid nitrogen to stabilize the water that is present in the conducting tissue. After filling with liquid nitrogen, discs are then collected from the frozen portion of the stem and stored in liquid nitrogen. The surface of disc is cleanly cut using a sliding microtome in a low temperature room at -20°C. Finally, the frozen sample is examined in a cryoscanning electron microscope after freeze-etching and metal coating.

元の言語英語
ホスト出版物のタイトルElectron Microscopy: Methods and Protocols
ページ497-506
ページ数10
369
DOI
出版物ステータス出版済み - 2 27 2007

出版物シリーズ

名前Methods in Molecular Biology
369
ISSN(印刷物)10643745

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Electron Microscopy
Nitrogen
Water
Freeze Etching
Metals
Electrons
Temperature

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

Utsumi, Y., & Sano, Y. (2007). Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy. : Electron Microscopy: Methods and Protocols (巻 369, pp. 497-506). (Methods in Molecular Biology; 巻数 369). https://doi.org/10.1385/1-59745-294-7:497

Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy. / Utsumi, Yasuhiro; Sano, Yuzou.

Electron Microscopy: Methods and Protocols. 巻 369 2007. p. 497-506 (Methods in Molecular Biology; 巻 369).

研究成果: 著書/レポートタイプへの貢献

Utsumi, Y & Sano, Y 2007, Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy. : Electron Microscopy: Methods and Protocols. 巻. 369, Methods in Molecular Biology, 巻. 369, pp. 497-506. https://doi.org/10.1385/1-59745-294-7:497
Utsumi Y, Sano Y. Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy. : Electron Microscopy: Methods and Protocols. 巻 369. 2007. p. 497-506. (Methods in Molecular Biology). https://doi.org/10.1385/1-59745-294-7:497
Utsumi, Yasuhiro ; Sano, Yuzou. / Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy. Electron Microscopy: Methods and Protocols. 巻 369 2007. pp. 497-506 (Methods in Molecular Biology).
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