Crystal structure of the overlapping dinucleosome composed of hexasome and octasome

Daiki Kato, Akihisa Osakabe, Yasuhiro Arimura, Yuka Mizukami, Naoki Horikoshi, Kazumi Saikusa, Satoko Akashi, Yoshifumi Nishimura, Sam Yong Park, Jumpei Nogami, Kazumitsu Maehara, Yasuyuki Ohkawa, Atsushi Matsumoto, Hidetoshi Kono, Rintaro Inoue, Masaaki Sugiyama, Hitoshi Kurumizaka

研究成果: Contribution to journalArticle

31 引用 (Scopus)

抜粋

Nucleosomes are dynamic entities that are repositioned along DNA by chromatin remodeling processes. A nucleosome repositioned by the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms the intermediate "overlapping dinucleosome." Here, we report the crystal structure of the overlapping dinucleosome, in which two nucleosomes are associated, at 3.14-angstrom resolution. In the overlapping dinucleosome structure, the unusual "hexasome" nucleosome, composed of the histone hexamer lacking one H2A-H2B dimer from the conventional histone octamer, contacts the canonical "octasome" nucleosome, and they intimately associate. Consequently, about 250 base pairs of DNA are left-handedly wrapped in three turns, without a linker DNA segment between the hexasome and octasome moieties. The overlapping dinucleosome structure may provide important information to understand how nucleosome repositioning occurs during the chromatin remodeling process.

元の言語英語
記事番号356
ページ(範囲)205-208
ページ数4
ジャーナルScience
356
発行部数6334
DOI
出版物ステータス出版済み - 4 14 2017

All Science Journal Classification (ASJC) codes

  • General

フィンガープリント Crystal structure of the overlapping dinucleosome composed of hexasome and octasome' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Kato, D., Osakabe, A., Arimura, Y., Mizukami, Y., Horikoshi, N., Saikusa, K., Akashi, S., Nishimura, Y., Park, S. Y., Nogami, J., Maehara, K., Ohkawa, Y., Matsumoto, A., Kono, H., Inoue, R., Sugiyama, M., & Kurumizaka, H. (2017). Crystal structure of the overlapping dinucleosome composed of hexasome and octasome. Science, 356(6334), 205-208. [356]. https://doi.org/10.1126/science.aak9867