Crystal structure of the PX domain of Vps17p from Saccharomyces cerevisiae

Takayuki Obita, Koji Inaka, Daisuke Kohda, Nobuo Maita

研究成果: ジャーナルへの寄稿学術誌査読

抄録

The structure determination of the PX (phox homology) domain of the Saccharomyces cerevisiae Vps17p protein presented a challenging case for molecular replacement because it has noncrystallographic symmetry close to a crystallographic axis. The combination of diffraction-quality crystals grown under microgravity on the International Space Station and a highly accurate template structure predicted by AlphaFold2 provided the key to successful crystal structure determination. Although the structure of the Vps17p PX domain is seen in many PX domains, no basic residues are found around the canonical phosphatidylinositol phosphate (PtdIns-P) binding site, suggesting an inability to bind PtdIns-P molecules.

本文言語英語
ページ(範囲)210-216
ページ数7
ジャーナルActa Crystallographica Section F: Structural Biology Communications
78
DOI
出版ステータス出版済み - 5月 1 2022

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 遺伝学
  • 凝縮系物理学

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